AAV5 VP1 抗体 (AbBy Fluor® 594)
Quick Overview for AAV5 VP1 抗体 (AbBy Fluor® 594) (ABIN2802173)
抗原
适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody will recognize many AAV strains, including AAV5, AAV4, AAV3B, AAV9, and AAV13
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交叉反应
- Virus
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交叉反应 （详细）
- AAV5
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic peptide derived from Adeno-Associated Virus 5 capsid protein VP1
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亚型
- IgG
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应用备注
- IF(IHC-P) 1:50-200
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
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有效期
- 12 months
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- AAV5 VP1 (Adeno-Associated Virus 5, VP1 (AAV5 VP1))
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别名
- AAV5 Capsid Protein VP1
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物质类
- Viral Protein
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背景
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Synonyms: capsid protein Adeno-associated 5 virus, capsid protein [Adeno-associated virus - 5], capsid protein AAV5, Parvovirus coat protein VP1, capsid protein.
Background: Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus
抗原
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