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SYK 抗体 (AA 314-339)

SYK 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 4D10 unconjugated
产品编号 ABIN967352
发货至: 中国
  • 抗原 See all SYK 抗体
    SYK (Spleen tyrosine Kinase (SYK))
    抗原表位
    • 29
    • 22
    • 20
    • 13
    • 10
    • 10
    • 8
    • 8
    • 8
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 314-339
    适用
    • 170
    • 82
    • 51
    • 11
    • 5
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    宿主
    • 158
    • 15
    • 3
    小鼠
    克隆类型
    • 161
    • 14
    单克隆
    标记
    • 102
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    This SYK antibody is un-conjugated
    应用范围
    • 147
    • 84
    • 46
    • 26
    • 23
    • 19
    • 14
    • 14
    • 13
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS)
    品牌
    BD Pharmingen™
    产品特性
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Human Syk peptide aa. 314-339
    克隆位点
    4D10
    亚型
    IgG2a
    Top Product
    Discover our top product SYK Primary Antibody
  • 应用备注
    Western Blot: EB1 control lysate [50 µg (1 µg/ml)] is provided as a positive control (store lysate at -20°C).
    Additional application: The FITC-conjugated format of clone 4D10 is useful in immunofluorescent staining and flow cytometric analysis.
    说明

    Related Products: ABIN967389

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    SDS-PAGE buffer (62 mM Tris pH 6.8, 2 % SDS, 0.9 % b-mercaptoethanol, 0.003 % bromophenol blue, 5 % glycerol)
    储存条件
    -20 °C
    储存方法
    Store undiluted at -20°C.
  • Latour, Veillette: "Proximal protein tyrosine kinases in immunoreceptor signaling." in: Current opinion in immunology, Vol. 13, Issue 3, pp. 299-306, (2001) (PubMed).

    Turner, Schweighoffer, Colucci, Di Santo, Tybulewicz: "Tyrosine kinase SYK: essential functions for immunoreceptor signalling." in: Immunology today, Vol. 21, Issue 3, pp. 148-54, (2000) (PubMed).

    Chu, van Oers, Malissen, Harris, Elder, Weiss: "Pre-T cell receptor signals are responsible for the down-regulation of Syk protein tyrosine kinase expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2610-20, (1999) (PubMed).

    Costello, Turner, Walters, Cunningham, Bauer, Downward, Tybulewicz: "Critical role for the tyrosine kinase Syk in signalling through the high affinity IgE receptor of mast cells." in: Oncogene, Vol. 13, Issue 12, pp. 2595-605, (1997) (PubMed).

  • 抗原
    SYK (Spleen tyrosine Kinase (SYK))
    别名
    Syk (SYK 产品)
    别名
    p72-Syk antibody, Sykb antibody, p72syk antibody, spleen associated tyrosine kinase antibody, spleen tyrosine kinase antibody, syk antibody, SYK antibody, Syk antibody
    背景
    Syk is a member of the family of non-receptor type protein-tyrosine kinases and plays a crucial role in lymphocyte signaling and development. Syk is expressed in all haematopoietic cells and contributes to the signal transduction process by binding to a tyrosine-based activation motif (ITAM) of immune receptors, including Igalpha, TCRzeta, CD3epsilon, FcepsilonRIbeta, and FceRIgamma. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains thereby activating Syk and causing tyrosines in Syk to undergo auto-phosphorylation or phosphorylation. These phosphorylated sites can act as binding sites for other signaling molecules or help to regulate enzymatic activity. For example, in mast cells, Syk can activate downstream targets such as phospholipase Cgamma1 and VAV. Studies in Syk-/- mast cells identified defects in both the ERK-MAP and JNK-MAP kinase pathways. Syk migrates as 72 kDa in SDS/PAGE. (Predicted MW from SWISS-PROT is 72 kDa, Accession number P43405).
    途径
    Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling
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