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MAP1LC3A 抗体 (cleaved)

This 兔多克隆 antibody specifically detects MAP1LC3A in WB, IF, ICC 和 IHC (p). It exhibits reactivity toward 人和小鼠. It has been mentioned in 35+ publications

Immunofluorescent analysis of 4 % paraforldehyde-fixed, 0. (MAP1LC3A 抗体 (cleaved))

Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100 ng/mL, using Cleaved-G8a (M1LC3A) 1805a (upper) or Beta-actin (lower). (MAP1LC3A 抗体 (cleaved))

Western blot analysis of lysates from NIH/3T3 cells, untreated or treated with chloroquine, using Cleaved-G8a (M1LC3A) 1805a (upper) or Beta-actin (lower). (MAP1LC3A 抗体 (cleaved))

Western blot analysis of lysates from NIH/3T3 cells, untreated or treated with chloroquine, using Cleaved-G8a (M1LC3A)(R)(upper) or Beta-actin (lower). (MAP1LC3A 抗体 (cleaved))

Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100 ng/mL, using Cleaved-G8a (M1LC3A) Antibody 1805a (upper) or Beta-actin (lower). (MAP1LC3A 抗体 (cleaved))

7 images

(35 references)

产品编号 ABIN388483

发货至: 中国

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Quick Overview for MAP1LC3A 抗体 (cleaved) (ABIN388483)

抗原

See all MAP1LC3A 抗体

MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

适用

人, 小鼠

宿主

兔

克隆类型

多克隆

标记

This MAP1LC3A antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

RB38908

抗原表位
20

15

12

10

8

7

7

6

6

5

5

5

4

4

4

2

2

2

2

2

2

2

2

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2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 89-120, cleaved

预测反应

Zf, B, Rat

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This Cleaved LC3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-120 amino acids from human Cleaved LC3A or LC3B.

亚型

IgG
anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term) antibody
MAP1LC3A 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7481 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-121) antibody
MAP1LC3A 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5B10D8 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 77-106), (C-Term) antibody
MAP1LC3A 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB7485 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term) antibody
MAP1LC3A 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7609 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-120) antibody
MAP1LC3A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-121) antibody
MAP1LC3A 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term), (pSer12) antibody
MAP1LC3A 适用: 人 WB, DB, EIA 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) antibody
MAP1LC3A 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), EIA 宿主: 小鼠 Monoclonal 166AT1234 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 30-56) antibody
MAP1LC3A 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB11840 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) antibody
MAP1LC3A 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated

应用备注

IF: 1:25. IF: 1:25. WB: 1:500. WB: 1:500. WB: 1:500. WB: 1:500. WB: 1:1000

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze-thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

有效期

6 months
Giatromanolaki, Sivridis, Kalamida, Koukourakis: "Transcription Factor EB Expression in Early Breast Cancer Relates to Lysosomal/Autophagosomal Markers and Prognosis." in: Clinical breast cancer, Vol. 17, Issue 3, pp. e119-e125, (2018) (PubMed).

Tavera-Mendoza, Westerling, Libby, Marusyk, Cato, Cassani, Cameron, Ficarro, Marto, Klawitter, Brown: "Vitamin D receptor regulates autophagy in the normal mammary gland and in luminal breast cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 11, pp. E2186-E2194, (2018) (PubMed).

Bingel, Koeneke, Ridinger, Bittmann, Sill, Peterziel, Wrobel, Rettig, Milde, Fernekorn, Weise, Schober, Witt, Oehme: "Three-dimensional tumor cell growth stimulates autophagic flux and recapitulates chemotherapy resistance." in: Cell death & disease, Vol. 8, Issue 8, pp. e3013, (2018) (PubMed).

Miyamoto, Takano, Aoyama, Soyama, Yoshikawa, Tsuda, Furuya: "Inhibition of autophagy protein LC3A as a therapeutic target in ovarian clear cell carcinomas." in: Journal of gynecologic oncology, Vol. 28, Issue 3, pp. e33, (2017) (PubMed).

Martinet, Roth, De Meyer: "Standard Immunohistochemical Assays to Assess Autophagy in Mammalian Tissue." in: Cells, Vol. 6, Issue 3, (2017) (PubMed).

Shrestha, Assani, Rinehardt, Albastroiu, Zhang, Shell, Amer, Schlesinger, Kopp: "Cysteamine-mediated clearance of antibiotic-resistant pathogens in human cystic fibrosis macrophages." in: PLoS ONE, Vol. 12, Issue 10, pp. e0186169, (2017) (PubMed).

Giatromanolaki, Kalamida, Sivridis, Karagounis, Gatter, Harris, Koukourakis: "Increased expression of transcription factor EB (TFEB) is associated with autophagy, migratory phenotype and poor prognosis in non-small cell lung cancer." in: Lung cancer (Amsterdam, Netherlands), Vol. 90, Issue 1, pp. 98-105, (2016) (PubMed).

Tanaka, Whelan, Chandramouleeswaran, Kagawa, Rustgi, Noguchi, Guha, Srinivasan, Amanuma, Ohashi, Muto, Klein-Szanto, Noguchi, Avadhani, Nakagawa: "ALDH2 modulates autophagy flux to regulate acetaldehyde-mediated toxicity thresholds." in: American journal of cancer research, Vol. 6, Issue 4, pp. 781-96, (2016) (PubMed).

Vanoli, Necchi, Barozzi, Manca, Pecci, Solcia: "Chaperone molecules concentrate together with the ubiquitin-proteasome system inside particulate cytoplasmic structures: possible role in metabolism of misfolded proteins." in: Histochemistry and cell biology, Vol. 144, Issue 2, pp. 179-84, (2015) (PubMed).

Pecci, Necchi, Barozzi, Vitali, Boveri, Elena, Bernasconi, Noris, Solcia: "Particulate cytoplasmic structures with high concentration of ubiquitin-proteasome accumulate in myeloid neoplasms." in: Journal of hematology & oncology, Vol. 8, pp. 71, (2015) (PubMed).

Kong, Whelan, Laczkó, Dang, Caro Monroig, Soroush, Falcone, Amaravadi, Rustgi, Ginsberg, Falk, Nakagawa, Lynch: "Autophagy levels are elevated in barrett's esophagus and promote cell survival from acid and oxidative stress." in: Molecular carcinogenesis, (2015) (PubMed).

Assani, Tazi, Amer, Kopp: "IFN-? stimulates autophagy-mediated clearance of Burkholderia cenocepacia in human cystic fibrosis macrophages." in: PLoS ONE, Vol. 9, Issue 5, pp. e96681, (2014) (PubMed).

Armani, Cinti, Marzolla, Morgan, Cranston, Antelmi, Carpinelli, Canese, Pagotto, Quarta, Malorni, Matarrese, Marconi, Fabbri, Rosano, Cinti, Young, Caprio: "Mineralocorticoid receptor antagonism induces browning of white adipose tissue through impairment of autophagy and prevents adipocyte dysfunction in high-fat-diet-fed mice." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 8, pp. 3745-57, (2014) (PubMed).

Liao, Sun, Wang, Huang, Liu, Liao, Shi: "LC3A-positive "stone-like" structures predict an adverse prognosis of gastric cancer." in: Anatomical record (Hoboken, N.J. : 2007), Vol. 297, Issue 4, pp. 653-62, (2014) (PubMed).

Sivridis, Koukourakis, Mendrinos, Touloupidis, Giatromanolaki: "Patterns of autophagy in urothelial cell carcinomas--the significance of "stone-like" structures (SLS) in transurethral resection biopsies." in: Urologic oncology, Vol. 31, Issue 7, pp. 1254-60, (2013) (PubMed).

Koukourakis, Giatromanolaki, Zois, Sivridis: "LC3 immunostaining pitfalls." in: Histopathology, Vol. 62, Issue 6, pp. 962-3, (2013) (PubMed).

Giatromanolaki, Koukourakis, Pouliliou, Gatter, Pezzella, Harris, Sivridis: "Overexpression of LC3A autophagy protein in follicular and diffuse large B-cell lymphomas." in: Hematology/oncology and stem cell therapy, Vol. 6, Issue 1, pp. 20-5, (2013) (PubMed).

Radtke, English, Rondeau, Leib, Lippé, Desjardins: "Inhibition of the host translation shutoff response by herpes simplex virus 1 triggers nuclear envelope-derived autophagy." in: Journal of virology, Vol. 87, Issue 7, pp. 3990-7, (2013) (PubMed).

Abdulrahman, Khweek, Akhter, Caution, Tazi, Hassan, Zhang, Rowland, Malhotra, Aeffner, Davis, Valvano, Amer: "Depletion of the ubiquitin-binding adaptor molecule SQSTM1/p62 from macrophages harboring cftr ?F508 mutation improves the delivery of Burkholderia cenocepacia to the autophagic machinery." in: The Journal of biological chemistry, Vol. 288, Issue 3, pp. 2049-58, (2013) (PubMed).

Giatromanolaki, Sivridis, Mendrinos, Koutsopoulos, Koukourakis: "Autophagy proteins in prostate cancer: relation with anaerobic metabolism and Gleason score." in: Urologic oncology, Vol. 32, Issue 1, pp. 39.e11-8, (2013) (PubMed).

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抗原

MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

别名

LC3A

背景

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

基因ID

84557

NCBI登录号

NP_115903, NP_852610

UniProt

Q9H492, Q9GZQ8

途径

Autophagy