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Cytokeratin 5 抗体 (Biotin)

KRT5 适用: 人, 小鼠, Pig, Cow, Amphibian, Hydra IF, FACS, IHC (p), IHC (fro), EIA 宿主: 小鼠 Monoclonal C22 Biotin
产品编号 ABIN112168
发货至: 中国
  • 抗原 See all Cytokeratin 5 (KRT5) 抗体
    Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))
    适用
    • 119
    • 75
    • 49
    • 12
    • 9
    • 9
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    人, 小鼠, Pig, Cow, Amphibian, Hydra
    宿主
    • 86
    • 43
    • 1
    小鼠
    克隆类型
    • 72
    • 58
    单克隆
    标记
    • 64
    • 14
    • 10
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Cytokeratin 5 antibody is conjugated to Biotin
    应用范围
    • 91
    • 51
    • 45
    • 32
    • 25
    • 21
    • 16
    • 14
    • 13
    • 7
    • 7
    • 5
    • 5
    • 5
    • 2
    • 2
    • 1
    Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    特异性
    Antibody clone C22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with Keratins K5 and K8 present in nearly all epithelia. Polypeptide Reacting: Mr 52 500, Mr 58 000 keratins (type II keratins K5 and K8, formerly also designated cytokeratins 5 and 8) of human epithelial cells. Reactivities on Cultured Cell Lines (tested so far): MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK. Epitope has been mapped to aa 353-367 on alpha helical rod domain (See Waseem et al., 2004).
    交叉反应 (详细)
    Also reacts to: KRT8
    纯化方法
    Affinity Chromatography on Protein A
    免疫原
    Human keratin K8, purified from SDS PAGE gel
    克隆位点
    C22
    亚型
    IgG1
    Top Product
    Discover our top product KRT5 Primary Antibody
  • 应用备注
    ELISA. Flow Cytometry. Immunocytology. Immunohistochemistry on Frozen Tissues (1/10-1/20), Incubation time: 1 h at RT. Immunohistochemistry on Paraffin-Embedded Tissues (1/10-1/20), Incubation time: overnight at 2-8 °C. With paraffin-embedded sections, protease pretreatment is requiredprior to antibody application.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    缓冲液
    0.09 % Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原
    Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))
    别名
    Cytokeratin 5 (KRT5 产品)
    别名
    3300001P10Rik antibody, AW146334 antibody, CK5 antibody, K5 antibody, Krt2-5 antibody, Tfip8 antibody, DDD antibody, DDD1 antibody, EBS2 antibody, KRT5A antibody, Sak57 antibody, ckii antibody, wu:fb02h09 antibody, wu:fj02d02 antibody, wu:fk78b12 antibody, zfCKII antibody, KRT75 antibody, CK-5 antibody, Keratin-5 antibody, keratin 5 antibody, Krt5 antibody, KRT5 antibody, krt5 antibody
    背景
    Cytokeratin 5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.Synonyms: 58 kDa Cytokeratin, CK5, Cytokeratin-5, K5, KRT5, Keratin 5, Keratin type II cytoskeletal 5, Keratin-5
    基因ID
    3852
    NCBI登录号
    NP_000415
    UniProt
    P13647
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