Ly6C 抗体 (Biotin)
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Quick Overview for Ly6C 抗体 (Biotin) (ABIN111873)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This Monoclonl antibody ER-MP20 is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is ideally suitable for the detection of monocytes in bone marrow samples by FACS. ER-MP20 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 (anti F4/80 antibody BM4007) is recommended. ER-MP20 also detects a wide range of endothelial cells. Antigen Distribution on Isolated cells: In bone marrow cells the antigen is found on monoblasts and late CFU-M cells as well as on monocytes. It is also found on granulocytes and a subpopulation of lymphocytes in the peripheral blood. Granulocytic cells show a dull, and monocytic cells a bright antigen surface expression. Lymphoid cells express the antigen only very weakly. Thus, in the bone marrow three useful FACS windows can be defined for cell sorting purposes. Antigen Distribution on Tissue Sections: The antigen is found on macrophage precursor subpopulations in the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Endothelial cells of small vessels in various organs also stain positive with ER-MP20. Activated macrophages in inflammatory tissues also express the ER-MP20-related antigen.
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交叉反应 （详细）
- Species reactivity (tested):Mouse (Macrophage precursor cells, endothelial cells).
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纯化方法
- Affinity Chromatography
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免疫原
- Mouse macrophage cell lines. Remarks: Antigen/Epitope: The antigen is a glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant 14kD surface protein which is very similar to Ly-6C and may be analogous to Human CD59. It is inducible by IFN-alpha, IFN-beta and IFN-gamma.
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亚型
- IgG2a
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anti-Ly-6C (Ly-6C) antibody
Ly-6C 适用: 小鼠 FACS, IHC (fro), Act 宿主: 大鼠 Monoclonal HK1-4 unconjugated
anti-Ly-6C (Ly-6C) antibodyLy-6C 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Ly-6C (Ly-6C) antibody (PE)Ly-6C 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal HK1-4 PE
anti-Ly-6C (Ly-6C) antibody (FITC)Ly-6C 适用: 小鼠 FACS, IHC (fro) 宿主: 大鼠 Monoclonal HK1-4 FITC
anti-Ly-6C (Ly-6C) antibody (Biotin)Ly-6C 适用: 小鼠 FACS, IHC (fro) 宿主: 大鼠 Monoclonal HK1-4 Biotin
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应用备注
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Immunohistochemistry on Frozen Sections: 0.2 μg/mL (1/1000)Immunohistochemistry on Paraffin Sections: 0.4 μg/mL (1/500). Proteinase K pretreatmentfor antigen retrieval is recommended. Suggested Positive Control: Mouse spleen. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.5 mL distilled water.
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浓度
- 0.2 mg/mL
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缓冲液
- Stock solution containing PBS, pH 7.2 with 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2-8 °C for 2 months or (in aliquots) at -20 °C for longer.
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- Ly6C (Ly-6C) (Ly-6C)
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别名
- Ly6c
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背景
- Ly-6C is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T-cell subsets. Mice with the Ly-6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly-6C+ and CD8+Ly-6C+ cells, while Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly-6C+ lymphocytes. Ly-6C may play a role in the development and maturation of lymphocytes.Synonyms: Ly-6C2, Ly6C2, Lymphocyte antigen 6C2, Mouse Macrophage Marker
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基因ID
- 100041546
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NCBI登录号
- NP_001092687
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UniProt
- P09568
抗原
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