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Ly-6C 抗体

ABIN111872 产品详细信息, 供应商: Log in to see
抗原
  • AA682074
  • AA959465
  • Ly-6C
  • Ly-6C1
  • Ly6c
  • lymphocyte antigen 6 complex, locus C1
  • Ly6c1
适用
小鼠
98
宿主
大鼠
97
1
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
14
14
10
5
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
93
25
23
9
4
4
3
2
2
1
1
1
1
1
Supplier
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免疫原 Mouse macrophage cell lines Remarks: Antigen/Epitope: The antigen is a glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant 14kD surface protein which is very similar to Ly-6C and may be analogous to Human CD59. It is inducible by IFN-alpha, IFN-beta and IFN-gamma.
克隆位点 ER-MP20
亚型 IgG2a
特异性 This Monoclonl antibody ER-MP20 is useful for the detection of macrophage precursor cells in the mid-stage development stage (late CFU-M, monoblasts and monocytes). It is ideally suitable for the detection of monocytes in bone marrow samples by FACS. ER-MP20 also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan-macrophage marker BM8 (anti F4/80 antibody BM4007) is recommended. ER-MP20 also detects a wide range of endothelial cells. Antigen Distribution on Isolated cells: In bone marrow cells the antigen is found on monoblasts and late CFU-M cells as well as on monocytes. It is also found on granulocytes and a subpopulation of lymphocytes in the peripheral blood. Granulocytic cells show a dull, and monocytic cells a bright antigen surface expression. Lymphoid cells express the antigen only very weakly. Thus, in the bone marrow three useful FACS windows can be defined for cell sorting purposes. Antigen Distribution on Tissue Sections: The antigen is found on macrophage precursor subpopulations in the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Endothelial cells of small vessels in various organs also stain positive with ER-MP20. Activated macrophages in inflammatory tissues also express the ER-MP20-related antigen.
交叉反应 (详细) Species reactivity (tested):Mouse (Macrophage precursor cells).
纯化方法 Affinity Chromatography
别名 Ly6c
背景 Ly-6C is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T-cell subsets. Mice with the Ly-6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly-6C+ and CD8+Ly-6C+ cells, while Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly-6C+ lymphocytes. Ly-6C may play a role in the development and maturation of lymphocytes.Synonyms: Ly-6C2, Ly6C2, Lymphocyte antigen 6C2, Mouse Macrophage Marker
基因ID 100041546
NCBI登录号 NP_001092687
UniProt P09568
应用备注 Immunohistochemistry on Frozen Sections: 0.5 μg/mL (1/400)Immunohistochemistry on Paraffin Sections: 1 μg/mL (1/200). Proteinase K pretreatmentfor antigen retrieval is recommended. Suggested Positive Control: Mouse spleen. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程 Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS
限制 仅限研究用
溶解方式 Restore with 0.5 mL distilled water.
浓度 0.2 mg/mL
缓冲液 Stock solution containing PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
储存液 Thimerosal (Merthiolate)
注意事项 This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid prolonged exposure to light. Avoid freeze/thaw cycles.
厂商提供的图像
Immunohistochemistry (IHC) image for anti-Ly-6C 抗体 (ABIN111872) Figure 1. Immunohistochemistry on Mouse spleen frozen sections using antibody ABIN111...
背景 McCormack, Leenen, Walker: "Macrophage progenitors from mouse bone marrow and spleen differ in their expression of the Ly-6C differentiation antigen." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 6389-98, 1994 (PubMed).

de Bruijn, Slieker, van der Loo, Voerman, van Ewijk, Leenen: "Distinct mouse bone marrow macrophage precursors identified by differential expression of ER-MP12 and ER-MP20 antigens." in: European journal of immunology, Vol. 24, Issue 10, pp. 2279-84, 1994 (PubMed).

Leenen, Melis, Slieker, Van Ewijk: "Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens." in: European journal of immunology, Vol. 20, Issue 1, pp. 27-34, 1990 (PubMed).

Leenen, Slieker, Melis, Van Ewijk: "Murine macrophage precursor characterization. I. Production, phenotype and differentiation of macrophage precursor hybrids." in: European journal of immunology, Vol. 20, Issue 1, pp. 15-25, 1990 (PubMed).