Recombinant 兔 anti-人 IgA Antibody
IHC
Monoclonal
MSVA-700R
IgG
Recombinant Antibody
unconjugated
Quick Overview for Recombinant 兔 anti-人 IgA Antibody (ABIN7477497)
抗原
IgA
抗体类型
Recombinant Antibody
克隆类型
单克隆
应用范围
Immunohistochemistry (IHC)
克隆位点
MSVA-700R
亚型
IgG
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适用
- 人
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宿主
- 兔
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Rabbit anti-Human IgA (Chain alpha), (Heavy Chain) antibody
ELISA, WB, IHC unconjugated
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Tonsil: A strong staining should be seen in a fraction of plasma cells.
Negative Control: Tonsil: Lymphocytes should not stain for IgA.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- IgA
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物质类
- Antibody
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背景
- A2m Marker, Ig alpha 1 Chain C Region, Ig alpha 2 Chain C Region, IGHA1, IGHA2, Immunoglobulin Am1, Immunoglobulin Am2, Immunoglobulin Heavy Constant Alpha 1, Immunoglobulin Heavy Constant Alpha 2,IgA antibody validated for immunohistochemistry on 76 different Normal Tissues
抗原
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