山羊 anti-人 kappa Light Chain Antibody (Texas Red (TR))
Rockland 609-1910
FACS, FM, FLISA
Polyclonal
IgG
Texas Red (TR)
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- 抗原 See all kappa Light Chain products
- kappa Light Chain
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Texas Red (TR)
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应用范围
- Flow Cytometry (FACS), Fluorescence Microscopy (FM), FLISA
- Supplier Product No.
- 609-1910
- Supplier
- Rockland
- 原理
- Human (kappa chain) Antibody Texas Red™ Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. Solid phase adsorption(s) against a panel of Human IgG Lambda, IgM Lambda and IgA Lambda proteins was performed. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
- 产品特性
- Anti-Human IgM (mu heavy chain) Texas Red generated in goat detects specifically Human IgM mu heavy chain.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 免疫原
- Optional[Immunogen]: Human kappa light chain
- 亚型
- IgG
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Goat anti-Human kappa Light Chain antibody (FITC)
FACS, WB, ELISA, FM, DB, FLISA Polyclonal IgG FITC F(ab')2 fragment
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- 应用备注
- Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- kappa Light Chain
- Abstract
- kappa Light Chain 产品
- 背景
- The anti-Human κ (kappa chain) Antibody detects the kappa chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG kappa is conjugated to Texas Red™.
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