ELISA, Immunofluorescence (IF), Western Blotting (WB)
特异性
The reactivity of the antiserum is restricted to the IgD molecule as tested in ELISA, immunoblotting, immunoprecipitation. Precipitation takes place under special test conditions in the presence of polyethylene glycol. Monoclonal mouse antibody to human IgD, isotype specific
To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining of cytoplasmic IgD and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100, in ELISA from 1:25 upwards, in Western blotting from 1:50 upwards. These data should be interpreted as general recommendations only.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.
浓度
1 mg/mL
缓冲液
No foreign proteins added.
注意事项
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
储存条件
4 °C/-20 °C
储存方法
The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.