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小鼠 anti-人 IgE (Fc Region) Antibody

ELISA, WB, FACS, Func Monoclonal Le27-NBS01 IgG1 kappa unconjugated

product purity: gel permeation chromatography (Superose 12/HR) (小鼠 anti-人 IgE (Fc Region) Antibody)

Target binding kinetics (Biacore (小鼠 anti-人 IgE (Fc Region) Antibody)

2 images

(1 reference)

产品编号 ABIN363694

发货至: 中国

Quick Overview for 小鼠 anti-人 IgE (Fc Region) Antibody (ABIN363694)

抗原

IgE

克隆类型

单克隆

应用范围

63
51
36
28
20
17
15
14
10
9
8
6
5
3
3
3
1
1
1

ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

克隆位点

Le27-NBS01

亚型

IgG1 kappa

抗原表位
30

20

19

3

1

1

1

1

1

1

1
Fc Region
适用
51

31

15

9

6

5

4

2

1

1
人
宿主
59

25

16

11

4

1
小鼠

特异性

Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes

产品特性

IGHE, chromosome 14q32.33

纯化方法

Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B

纯度

> 95 %, tested by analytical Gel Permeation Chromatography

过滤

Sterile filtered

免疫原

purified human myeloma IgE
Mouse anti-Human IgE (Fc Region) antibody (Biotin)
ELISA Monoclonal HP6029 IgG2a Biotin

Goat anti-Human IgE antibody (Alkaline Phosphatase (AP))
ELISA, WB Polyclonal IgG Alkaline Phosphatase (AP)

Goat anti-Human IgE antibody
ELISA, WB Polyclonal IgG unconjugated

Goat anti-Human IgE antibody (FITC)
FACS Polyclonal IgG FITC

Goat anti-Human IgE antibody (Biotin)
ELISA, WB Polyclonal IgG Biotin

Goat anti-Human IgE antibody (HRP)
ELISA Polyclonal IgG HRP

Mouse anti-Human IgE (Fc Region) antibody (HRP)
ELISA, WB, IHC Monoclonal B3102E8 IgG1 HRP

Mouse anti-Human IgE (Fc Region) antibody (FITC)
ELISA, FLISA Monoclonal B3102E8 IgG1 FITC

Rabbit anti-Human IgE (Heavy Chain) antibody
ELISA, WB, IHC, ICC Polyclonal IgG unconjugated

Pig anti-Human IgE (Fc Region) antibody (FITC)
ELISA, IF, ICC, IHC (fro) Polyclonal FITC

应用备注

For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions less than 1:100 Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb Le27), tested by ELISA binding to immobilized human Standard IgE (SUS-11)

限制

仅限研究用
浓度

1 mg/mL

缓冲液

PBS, pH=7.2, sterile filtered, contains no NaN3

储存液

Azide free

注意事项

Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.

储存条件

4 °C
Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).
抗原

IgE

背景

The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.

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