小鼠 anti-鸭 IgY Antibody
ELISA, FACS, WB
Monoclonal
14A3
IgG1
unconjugated
Quick Overview for 小鼠 anti-鸭 IgY Antibody (ABIN305009)
抗原
IgY
克隆类型
单克隆
应用范围
ELISA, Flow Cytometry (FACS), Western Blotting (WB)
克隆位点
14A3
亚型
IgG1
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适用
- 鸭
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宿主
- 小鼠
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特异性
- Recognizes the light chain of duck immunoglobulin, shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes. Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1, revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). Recognizes IgA in Peking duck bile and can be used as a marker to identify B cells in both Peking and Muscovy duck and geese studies. Detects a band of approximately 25 kD in duck cell lysates in Western blotting.
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无交叉反应
- 小鸡
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产品特性
- Antigene is shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes. Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1, revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). Recognizes IgA in Peking duck bile and can be used as a marker to identify B cells in both Peking and Muscovy duck and geese studies. Detects a band of approximately 25 kDa in duck cell lysates in Western blotting.
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纯化方法
- Affinity purified
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免疫原
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Purified Peking duck yolk IgY.
Type of Immunogen: Purified protein
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应用备注
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Approved: ELISA, Flo, WB
Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. -
说明
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Target Species of Antibody: Duck
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, 0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
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储存条件
- -20 °C
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储存方法
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Short term: -20°C
Long term: -20°C.
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- IgY
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物质类
- Antibody
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分子量
- 25 kDa
抗原
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