产品编号 ABIN238423
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 小鼠 anti-Cow IgG Antibody (ABIN238423)
抗原
IgG
克隆类型
单克隆
应用范围
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
克隆位点
IVA285-1
亚型
IgG1
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适用
- Cow
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宿主
- 小鼠
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交叉反应 （详细）
- Species reactivity (tested):Bovine.
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纯化方法
- Protein A affinity chromatography
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免疫原
- Fraction of Bovine immunoglobulin.
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应用备注
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Western Blotting (Reducing conditions): 0,5-1 μg/mL, overnight in 4C. SDS-PAGE (12 % separating gel). Positive control and sample preparation: BS (bovine serum), dilution 1:50 in Laemmlireducing buffer, boiled in water bath for 3 min. Strongly reacts with bovine IgG light chains, weakly reacts with IgM. Flow Cytometry. Immunohistochemistry (frozen sections).
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, 15 mM Sodium Azide
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
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- IgG
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物质类
- Antibody
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背景
- Synonyms: Bovine Immunoglobulin G
抗原
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