山羊 anti-小鼠 IgG3 Antibody (FITC)
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- 抗原 See all IgG3 products
- IgG3
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), FLISA, Dot Blot (DB), Fluorescence Microscopy (FM)
- Supplier Product No.
- 610-102-043
- Supplier
- Rockland
- 原理
- Mouse IgG3 Antibody Fluorescein Conjugated Pre-adsorbed
- 交叉反应 （详细）
- Minimal crossreactivity against Bv, Hu, and Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.
- 产品特性
- Anti-Mouse IgG2b Fluorescein Antibody generated in goat detects reactivity to Mouse IgG2b (Gamma 2b chain).
- 纯化方法
- Anti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 免疫原
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Immunogen: Mouse IgG3 heavy chain
Immunogen Type: Native Protein
- 亚型
- IgG
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Goat anti-Mouse IgG3 antibody (HRP)
ELISA, FLISA Polyclonal IgG HRP
Goat anti-Mouse IgG3 (Heavy Chain) antibody (HRP)ELISA, WB, IHC Polyclonal IgG HRP
Goat anti-Mouse IgG3 antibodyELISA, FLISA Polyclonal IgG unconjugated
Goat anti-Mouse IgG3 (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Mouse IgG3 antibody (FITC)ELISA, FLISA Polyclonal IgG FITC
Rat anti-Mouse IgG3 antibodyELISA, FACS, FLISA Monoclonal SB76b IgG2a unconjugated
Goat anti-Mouse IgG3 (Heavy Chain) antibodyELISA, WB, IHC, FACS Polyclonal IgG unconjugated
Rat anti-Mouse IgG3 antibody (Biotin)ELISA, FACS, FLISA Monoclonal SB76b IgG2a Biotin
Goat anti-Mouse IgG3 antibody (Biotin) - PreadsorbedELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
Rat anti-Mouse IgG3 antibody (HRP)ELISA, FACS, FLISA Monoclonal SB76b IgG2a HRP
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- 应用备注
- Application Note: Anti-Mouse IgG3 Fluorescein antibody has been tested by dot blot and is suitable for Flow Cytometry, Immunohistochemistry and Immunofluorescence Microscopy, ELISA, western blotting as well as other anti IgG2b antibody based assays. Mouse IgG3 secondary antibody is available in a variety of formats. Flow Cytometry Dilution: 1:200 - 1:500 Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgG3
- Abstract
- IgG3 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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