山羊 anti-小鼠 IgM (Heavy Chain) Antibody
Quick Overview for 山羊 anti-小鼠 IgM (Heavy Chain) Antibody (ABIN1102423)
抗原
片段
克隆类型
应用范围
亚型
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抗原表位
- Heavy Chain
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适用
- 小鼠
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宿主
- 山羊
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特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes.
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纯化方法
- Immunoaffinity purified
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过滤
- Sterile filtered
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免疫原
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Anti-Mouse IgM was produced by repeated immunization with Mouse IgM heavy chain in goat.
Type of Immunogen: Purified protein
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Goat anti-Mouse IgM (Heavy Chain) antibody (TRITC)
ELISA, FACS, IHC Polyclonal IgG TRITC
Goat anti-Mouse IgM (Heavy Chain) antibody (FITC)ELISA, FACS, IHC Polyclonal IgG FITC
Goat anti-Mouse IgM (Heavy Chain) antibody (Biotin)ELISA, FACS, IHC, WB Polyclonal IgG Biotin
Goat anti-Mouse IgM (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Mouse IgM antibody (APC) - PreadsorbedELISA, FACS Polyclonal IgG APC
Goat anti-Mouse IgM antibody (PE) - PreadsorbedELISA, FACS Polyclonal IgG PE
Rat anti-Mouse IgM (Chain mu) antibody (HRP)ELISA, IHC Monoclonal SB73a IgG1 HRP
Goat anti-Mouse IgM (Heavy Chain) antibody (Cy5)FACS, IHC Polyclonal IgG Cy5
Rat anti-Mouse IgM antibody (PE)ELISA, FACS Monoclonal 1B4B1 IgG1 PE
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应用备注
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Approved: ELISA, Flo, IHC (1:1000 - 1:5000), WB (1:200 - 1:2000)
Usage: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10 x106 in flow cytometry is approximately 1.0 μg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide, sterile filtered
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- IgM
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物质类
- Antibody
抗原
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