IgE 蛋白
Quick Overview for IgE 蛋白 (ABIN7596472)
抗原
See all IgE 蛋白蛋白类型
宿主
资源
应用范围
纯度
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克隆类型
- Chimeric
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原理
- Chimeric Human IgE (JW8)
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特异性
- 4-Hydroxy-3-nitrophenylacetyl (NP)
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产品特性
- Recombinant chimeric IgE consisting of a human Fc heavy chain linked to the antigen binding variable region of a mouse hybridoma by in vitro DNA recombination. The antibody is specific for the hapten 4-Hydroxy-3-iodo-5-nitrophenylacetyl (NIP). IgE (JW8) recognizes the human high affinity IgE receptor (FceRIa) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with NIP-BSA conjugate as specific cross-linking agent.
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纯化方法
- Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B – mouse anti-human IgE mAb (BSW17)
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过滤
- Sterile filtered
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IgE (AA 208-429) protein (His-Avi Tag)
宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgE (AA 209-428) protein (His-Avi Tag)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 91-421) protein (His tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgE (AA 198-421) protein (His-Avi Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgE protein宿主: 人 宿主: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, Func, FACS
IgE protein (Biotin)宿主: 人 宿主: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, Func, FACS
IgE (AA 1-429) protein (His tag)宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, Imm, PC
IgE (AA 1-428) protein (His tag)宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
IgE (AA 1-432) protein (His tag)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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应用备注
- ELISA, Flow Cytometry, functional cellular assays (mast cell / basophil activation).
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说明
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100% reactivity, compared to calibrator (Internal Standard IgE (JW8)), tested by SPR (Biacore™) binding to immobilized recombinant human high affinity IgE receptor
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS) pH = 7.2
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储存液
- Azide free
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4 °C. For long term storage, aliquot and store at -20°C or -80°C. Avoid repeated freeze-thawing. Handle under sterile conditions, the mAb is formulated in sterile buffer solution and does not contain sodium azide as preservative. The product may be shipped on ice pack or dry ice.
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- IgE
抗原
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