IgG1 Fc (AA 98-324) 蛋白
-
- 抗原
- IgG1 Fc
- 蛋白类型
- Recombinant
- 产品特性
- AA 98-324
-
宿主
- 小鼠
-
资源
- HEK-293 Cells
- 原理
- Mouse IgG1 Fc Protein
- 序列
- Val98-Lys324
- 产品特性
- Recombinant Mouse IgG1 Fc Protein is expressed from HEK293 without tag.It contains Val98-Lys324.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 15.09 nM as determined in SPR assay (Biacore T200). See testing image for detail.
-
IgG1 Fc (AA 104-330) protein
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgG1 Fc protein (Fc Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
IgG1 Fc (AA 100-330) protein (DYKDDDDK Tag)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
IgG1 Fc (AA 100-330) protein (gD Tag)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
IgG1 Fc (AA 98-324) protein (AVI tag,Biotin)宿主: 小鼠 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- IgG1 Fc
- 背景
- Recombinant murine Fc fragment contains the hinge region, CH2, and CH3 region of the mouse IgG1 isotype. IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues.
- 分子量
- 25.7 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
- UniProt
- P01868
-
