CD28H (AA 23-150) protein (Fc Tag)
This Recombinant CD28H protein is expressed in HEK-293 Cells.
产品编号 ABIN7274253
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for CD28H (AA 23-150) protein (Fc Tag) (ABIN7274253)
抗原
CD28H
蛋白类型
Recombinant
宿主
人
资源
HEK-293 Cells
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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产品特性
- AA 23-150
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标记
- Fc Tag
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原理
- Human CD28H/IGPR-1 Protein
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序列
- Leu23-Gly150
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产品特性
- Recombinant Human CD28H/IGPR-1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu23-Gly150.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human B7-H7, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 81ng/ml determined by ELISA. See testing image for detail.
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CD28H (AA 23-150) protein (His tag)
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD28H (AA 23-150) (Active) protein (His tag)宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by reduced SDS-PAGE. Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- CD28H
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背景
- CD28H is constitutively expressed on all naive T cells. Repetitive antigenic exposure, however, induces a complete loss of CD28H on many T cells, and CD28H negative T cells have a phenotype of terminal differentiation and senescence. After extensive screening in a receptor array, a B7-like molecule, B7 homologue 5 (B7-H5), was identified as a specific ligand for CD28H.
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分子量
- 40.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
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UniProt
- Q96BF3-1
抗原
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