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CD28H (AA 23-150) protein (His tag)

This Recombinant protein is expressed in HEK-293 Cells.

Immobilized Human CD28H, His Tag at 2 μg/mL (100 μL/well) on the plate. (CD28H (AA 23-150) protein (His tag))

Human CD28H on Tris-Bis PAGE under reduced condition. (CD28H (AA 23-150) protein (His tag))

The purity of Human CD28H is greater than 95 % as determined by SEC-HPLC. (CD28H (AA 23-150) protein (His tag))

3 images

产品编号 ABIN7274250

发货至: 中国

Quick Overview for CD28H (AA 23-150) protein (His tag) (ABIN7274250)

抗原

CD28H

蛋白类型

Recombinant

宿主

人

资源

HEK-293 Cells

纯度

> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

产品特性

AA 23-150

标记

His tag

原理

Human CD28H/IGPR-1 Protein

序列

Leu23-Gly150

产品特性

Recombinant Human CD28H/IGPR-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Gly150.

过滤

0.22 μm filtered

内毒素水平

Less than 1EU per ug by the LAL method.

Biological Activity Comment

Immobilized Human CD28H, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 0.06μg/ml determined by ELISA. See testing image for detail.
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CD28H (AA 23-150) protein (Fc Tag)
宿主: 人宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

CD28H (AA 23-150) (Active) protein (His tag)
宿主: 人宿主: HEK-293 Cells Recombinant >90 % as determined by reduced SDS-PAGE. Active

CD28H (partial) protein (Fc Tag)
宿主: 人宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. WB

限制

仅限研究用
状态

Lyophilized

溶解方式

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

缓冲液

Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

储存条件

-20 °C,-80 °C

储存方法

-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

有效期

12 months
抗原

CD28H

背景

CD28H is constitutively expressed on all naive T cells. Repetitive antigenic exposure, however, induces a complete loss of CD28H on many T cells, and CD28H negative T cells have a phenotype of terminal differentiation and senescence. After extensive screening in a receptor array, a B7-like molecule, B7 homologue 5 (B7-H5), was identified as a specific ligand for CD28H.

分子量

15.1 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result.

UniProt

Q96BF3-1

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