HIV-1 Gag P15 蛋白
This Recombinant HIV-1 Gag P15 protein is expressed in 大肠杆菌（E. Coli） and has been mentioned in 3+ publications.
(3 references)
产品编号 ABIN2452192
发货至:
中国
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Quick Overview for HIV-1 Gag P15 蛋白 (ABIN2452192)
抗原
HIV-1 Gag P15
蛋白类型
Recombinant
宿主
Human Immunodeficiency Virus (HIV)
资源
大肠杆菌（E. Coli）
应用范围
Dot Blot (DB), SDS-PAGE (SDS), ELISA, Western Blotting (WB)
纯度
> 90 % by SDS-PAGE (CBB staining)
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应用备注
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1) It can be used as a substrate for HIV-1 protease in the presence of HIV-1 genomic RNA.
2) It can be used in studies of structure and function of AIDS virus as precursor of nucleocapsid p7 protein that binds to HIV-1 genome RNA.
3) It can be used as p15 antigen in detection of anti-HIV-1 p15 antibody in Western blotting or ELISA.
4) It can be used as a standard for the quantitative analysis of HIV-1 p15 antigen. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.42 mg/mL
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缓冲液
- 50 % glycerol, 20 mM Tris-HCl ( pH 7.5), 50 mM NaCl, 10 mM mercaptoethanol
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储存条件
- -20 °C
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: "HIV-1 gag proteins: diverse functions in the virus life cycle." in: Virology, Vol. 251, Issue 1, pp. 1-15, (1998) (PubMed).
: "Overproduction, purification, and diagnostic use of the recombinant HIV-1 Gag proteins, the precursor protein p55 and the processed products p17, p24, and p15." in: Microbiology and immunology, Vol. 39, Issue 7, pp. 473-83, (1996) (PubMed).
: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, (1986) (PubMed).
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: "HIV-1 gag proteins: diverse functions in the virus life cycle." in: Virology, Vol. 251, Issue 1, pp. 1-15, (1998) (PubMed).
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- HIV-1 Gag P15
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物质类
- Viral Protein
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背景
- HIV-1 Gag p15 is processed by digestion of its precursor Gag p55 by HIV-1 protease. This protein is further digested into nuclocapsid protein p7 and into p6 and p1 of unknown function. This digestion is promoted by binding of HIV-1 genome RNA and the two Zn finger motifs that exist in the p7 region. The produced nucleocapsid protein p7 regulates the RNA function by directly binding to HIV-1 genome RNA. HIV-1 Gag p15 was over-expressed as a recombinant protein in E. coli with a plasmid carrying the Gag p15 coding region of HIV-1 virus, subtype B, and highly purified by several steps of chromatography. Its molecular size is 15 kD, same as that of p15 purified from AIDS virus particles.
抗原
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