Chlamydia Pneumoniae IgG ELISA 试剂盒
This 人 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 .
Quick Overview for Chlamydia Pneumoniae IgG ELISA 试剂盒 (ABIN7666549)
抗原
Chlamydia Pneumoniae IgG
适用
人
检测方法
Colorimetric
实验类型
Indirect ELISA
应用范围
ELISA
样品类型
Plasma, Serum
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原理
- anti-Chlamydia pneumoniae IgG - ELISA kit for the detection of IgG antibodies to Chlamydia pneumoniae in human serum and plasma.
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品牌
- ELISA-VIDITEST
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Analytical Method
- Semi-Quantitative
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组件
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- ELISA break-away strips in the handling frame coated with the antigen STRIPS Ag 1 x 12 pcs
- 1.3 mL Negative control serum, r.t.u. NC 1 vial
- 1.3 mL Positive control serum, r.t.u. PC 1 vial
- 2.0 mL Calibrator, r.t.u. 1) CAL 1 vial
- 13 mL Anti-human IgG antibodies labelled with horseradish peroxidase (anti-IgG Px conjugate) r.t.u. CONJ 1 vial
- 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
- 60 mL Dilution buffer, r.t.u. DIL 1 vial
- 13 mL Chromogenic substrate TMB, r.t.u. TMB 1 vial
- 13 mL Stop solution, r.t.u. STOP 1 vial
- Instruction manual
- Certificate
- of quality 1) r.t.u., ready to use
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试剂未包括
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- Distilled or deionised water for dilution of buffer and standard concentrates
- appropriate and calibrated equipment for pipetting
- temperature controlled incubator
- spectrophotometer or platereader with the appropriate filters
- empty microtiter plate for pre-incubation
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应用备注
- Optimal working dilution should be determined by the investigator.
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板类型
- Pre-coated
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实验流程
- anti-Chlamydia pneumoniae IgG is a solid-phase immunoanalytical test. The purified, homogeneous native antigen is fixed to each well of the microtiterstrips. Specific antibodies present in the patient's sample are bound during the first incubation step. After removing unbound material by washing, the presence of the specific antibodies is detected using anti-human IgG conjugate during the second incubation. The unbound peroxidase conjugate is then removed by washing. TMB substrate is added, resulting in the development of a blue colour in positive samples. The enzyme reaction is terminated by addition of the stop solution (colour change to yellow). The intensity of the yellow colour thus developed is proportional to the concentration of antibodies in the sample. 2
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- The ELISA kit should be used within three months after opening. a. Store the kit and the kit reagents at +2 to +10°C, in a dry place and protected from the light. Under these conditions, the expiration period of the entire kit is indicated on the central label, the expiration date of the individual components is indicated on their package labels. b. Store unused strips in the sealable pouch and keep the desiccant inside. c. Kits are shipped in cooling bags, the transport time up to 72 hours have no influence on expiration. If you find damage at any part of the kit, please inform the manufacturer immediately. d. Store undiluted sera in aliquots at -18 to -28 °C. Frequent freezing and thawing is not recommended. Undiluted serum samples could be stored at +2 to +10°C up to one week. e. Do not store diluted samples. Always prepare fresh.
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- Chlamydia Pneumoniae IgG
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物质类
- Antibody
抗原
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