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- 抗原
- Thaumatin
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 最低检测浓度
- 80 ng/mL
- 应用范围
- ELISA
- 样品类型
- Food
- Analytical Method
- Quantitative
- 交叉反应 （详细）
- Cross reactivity: Acesufamat <0,1% Aspartam <0,1% Cycamat <0,1% Saccharin <0,1% Saccharose <0,1%
- 产品特性
- The determination of Thaumatin is carried out as direct sandwich ELISA.
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- 板类型
- Pre-coated
- 实验流程
- An antibody specific for Thaumatin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Thaumatin present is bound.. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the Thaumatin concentration
- 实验流程
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Incubation of samples/standards: 100 µL/well 2 h
Wash Incubation of detection antibody: 100 µL/well 2 h
Wash Substrate incubation: 100 µL/well 15 min
Add stop solution: 100 µL/well
Read at 450 nm - 实验精密度
- Intraassay precision (CV): (n=12) at 6 ng/ml: 6,2%
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
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- 抗原
- Thaumatin
- 背景
- The thaumatins is a mixture of proteins isolated from the katemfe fruit (Thaumatococcus daniellii Bennett). Some of the proteins in the thaumatin family are natural sweeteners roughly 2000 times more potent than sugar. Thaumatin has been approved as a sweetener in the European Union (E957), Israel, and Japan. In the United States, it is a Generally Recognized as Safe flavoring agent (FEMA GRAS 3732). The Thaumatin-ELISA is designed for the quantitative determination of Thaumatin in complex samples.
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