Quick Overview for RFP Western Blot Kit: for RFP Chemiluminescent Western Blotting (ABIN2669872)
抗原
RFP
(Red Fluorescent Protein (RFP))
适用
Discosoma
应用范围
Dot Blot (DB), Western Blotting (WB)
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克隆类型
- 多克隆
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Supplier Product No.
- kca379
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Supplier
- Rockland
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交叉反应
- Cherry, Tomato (Solanum lycopersicum), Banana, Orange, Plum, Strawberry
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Red Fluorescent Protein (RFP) ELISA Kit
RFP 适用: Discosoma Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate
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应用备注
- Use Anti-RFP Chemiluminescent Kit for Western Blotting for detection of RFP-tagged recombinant proteins by western blot. his kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.
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说明
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Detection Kit Type: Chemiluminescent Western Blot Kit
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限制
- 仅限研究用
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注意事项
- Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!
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储存条件
- RT/4 °C
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- RFP (Red Fluorescent Protein (RFP))
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别名
- DsRed Fluorescent Protein
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背景
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Red Fluorescent Protein (RFP) Western Bloting kit allows for the detection of RFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with optimized Anti-RFP antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
Synonyms: Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit, RFP, rRFP, mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry
抗原
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