DM1 Antibody Drug Conjugate (ADC) ELISA 试剂盒
This 人 not_set ELISA Kit specifically detects not_set
.
产品编号 ABIN2648224
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for DM1 Antibody Drug Conjugate (ADC) ELISA 试剂盒 (ABIN2648224)
抗原
DM1 Antibody Drug Conjugate (ADC)
适用
人
检测方法
Colorimetric
应用范围
ELISA
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灵敏度
- 0.0077 μg/mL
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产品特性
- DM1 Antibody Drug Conjugate (ADC) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for use in the quantitative determination of antibody-DM1-conjugate (Trastuzumab emtansine) level in test samples. It is useful for pre-clinical and clinical pharmacology study of DM1 Antibody Drug Conjugate (ADC).
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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实验时间
- 2.5 h
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实验流程
- Assay calibrators (antibody DM1 conjugate) and test serum samples are added directly to wells of a microtiter plate that is coated with specific anti-DM1 antibody. Subsequently, a horseradish peroxidase (HRP) conjugated DM1 is added to each well. During the incubation period, the antibody DM1 conjugate competes with the HRP conjugated DM1 for the limited binding sites of anti-DM1 antibody. An immune complex of well coated "anti-DM1 antibody - HRP conjugated DM1" is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction, which is terminated with an acidic reagent (i.e. ELISA stop solution). The absorbance is then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is inversely proportional to the amount of antibody-DM1 conjugate in the test sample. A calibration curve is generated by plotting the absorbance versus the respective antibody-DM1 conjugate concentration for each calibrator on a 4-parameter or log-logit curve fitting. The concentration of antibody-DM1 conjugate in test samples is determined directly from this calibration curve
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限制
- 仅限研究用
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储存条件
- 4 °C
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- DM1 Antibody Drug Conjugate (ADC)
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物质类
- Antibody
抗原
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