Quick Overview for HEK-293 Cells IgG4 同型对照 (ABIN2181283)
抗原
IgG4
应用范围
Isotype Control (IsoC)
亚型
IgG4
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适用
- 人
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宿主
- HEK-293 Cells
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产品特性
- This protein carries no "tag". The protein has a calculated MW of 25.8 kDa. As a result of glycosylation, the protein migrates as 33-35 kDa under reducing (R) condition, and 50-55 kDa under non-reducing (NR) condition (SDS-PAGE).
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纯度
- >95 % as determined by SDS-PAGE.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
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缓冲液
- 50 mM Tris, 100 mM Glycine, pH 7.5
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注意事项
- Avoid repeated freeze-thaw cycles.
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储存条件
- -20 °C
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储存方法
- No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
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- IgG4
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物质类
- Antibody
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背景
- Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).
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分子量
- 25.8 kDa
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UniProt
- P01861
抗原
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