S100A8/A9 Complex (Calprotectin) 抗体
Quick Overview for S100A8/A9 Complex (Calprotectin) 抗体 (ABIN966510)
抗原
See all S100A8/A9 Complex (Calprotectin) (S100A8/A9) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
 - This antibody reacts with macrophage L1 protein, also known as calprotectin, calgranulin, or cystic fibrosis antigen.
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纯化方法
 - Concentrated.
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免疫原
 - Affinity purified monocyte membrance preparation.
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亚型
 - IgG1
 
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应用备注
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                        Immunohistochemistry: 1:50-1:100.
Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. This antibody may be used at a dilution 1:50-1:100 in IHC. When using routinely processed tissues, predigestion with trypsin @ 1 mg/ml PBS, 10 mins. at 37 C. or a high temperature antigenic unmasking technique (boiling tissue sections in 10 mM citrate, pH 6.0 for 10-20 mins., folowed by cooling to RT for 10-20 mins.) is required. The optimal conditions should be determined by the individual laboratory. - 
                                            
说明
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Cellular Localization: cytoplasmic.
Recommended Positive Control: Tonsil, Lymph Node or Spleen - 
                                            
限制
 - 仅限研究用
 
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状态
 - Liquid
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浓度
 - 0.2 mg/ml.
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缓冲液
 - 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
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储存液
 - Sodium azide
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注意事项
 - This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
 - 4 °C
 
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                                            : "Acute kidney graft rejection. A morphological and immunohistological study on "zero-hour" and follow-up biopsies with special emphasis on cellular infiltrates and adhesion molecules."    in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 102, Issue 1, pp. 23-37, (1994)         (PubMed).        
 
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                                            : "Acute kidney graft rejection. A morphological and immunohistological study on "zero-hour" and follow-up biopsies with special emphasis on cellular infiltrates and adhesion molecules."    in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 102, Issue 1, pp. 23-37, (1994)         (PubMed).        
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- S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))
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别名
 - Macrophage/L 1 Protein/Calprotectin
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基因ID
 - 3552
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途径
 - S100 Proteins
 
抗原
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