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S100A8/A9 Complex (Calprotectin) 抗体

Cited in 1 publication. This 小鼠 单克隆 anti-S100A8/A9 Complex (Calprotectin) antibody (Clone B314-1 (MAC 387)) specifically detects S100A8/A9 Complex (Calprotectin) in IHC (p) 和 IHC (fro). The antibody is reactive with 人, Pig, Cat, 犬, 豚鼠, 猴, 兔, 大鼠, 小鼠 和 Baboon samples.
产品编号 ABIN966510
发货至: 中国
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Quick Overview for S100A8/A9 Complex (Calprotectin) 抗体 (ABIN966510)

抗原

See all S100A8/A9 Complex (Calprotectin) (S100A8/A9) 抗体
S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

适用

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人, Pig, Cat, 犬, 豚鼠, 猴, 兔, 大鼠, 小鼠, Baboon

宿主

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小鼠

克隆类型

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单克隆

标记

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This S100A8/A9 Complex (Calprotectin) antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

B314-1 (MAC 387)
  • 特异性

    This antibody reacts with macrophage L1 protein, also known as calprotectin, calgranulin, or cystic fibrosis antigen.

    纯化方法

    Concentrated.

    免疫原

    Affinity purified monocyte membrance preparation.

    亚型

    IgG1
  • 应用备注

    Immunohistochemistry: 1:50-1:100.
    Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. This antibody may be used at a dilution 1:50-1:100 in IHC. When using routinely processed tissues, predigestion with trypsin @ 1 mg/ml PBS, 10 mins. at 37 C. or a high temperature antigenic unmasking technique (boiling tissue sections in 10 mM citrate, pH 6.0 for 10-20 mins., folowed by cooling to RT for 10-20 mins.) is required. The optimal conditions should be determined by the individual laboratory.

    说明

    Cellular Localization: cytoplasmic.
    Recommended Positive Control: Tonsil, Lymph Node or Spleen

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/ml.

    缓冲液

    20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C
  • Andersen, Ladefoged, Larsen: "Acute kidney graft rejection. A morphological and immunohistological study on "zero-hour" and follow-up biopsies with special emphasis on cellular infiltrates and adhesion molecules." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 102, Issue 1, pp. 23-37, (1994) (PubMed).

  • 抗原

    S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

    别名

    Macrophage/L 1 Protein/Calprotectin

    基因ID

    3552

    途径

    S100 Proteins
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