CD22 抗体
Quick Overview for CD22 抗体 (ABIN965778)
抗原
See all CD22 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts strongly with CD22. CD22 is expressed on in all B cell cytoplasm. It appears as CD19 on the cell surface in developing B cells. It can also be found on the cell surface of most mature sIg+ B cells and on hairy cell and prolymphocytic leukemia cells. On frozen sections, CD22 is found in follicular mantle and marginal zone B cells. There is weak reaction in germinal center B cells. This antibody can be used for typing of leukemia, assigning B cell lineage, and in flow cytometry to identify mature B cells. Also, it can be used to study B cell adhesion and to identify B cell lymphomas.
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纯化方法
- Concentrated.
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免疫原
- Raji (Burkitt's lymphoma) cells.
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亚型
- IgG1
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应用备注
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Immunohistochemistry: 1:50-1:100 on frozen tissue only.
Flow cytometry: 1:150-1:300.
Staining Procedure: This antibody can be used on frozen tissue only. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory. -
说明
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Recommended Positive Control: Tonsil, Spleen, Thymus, Lymph Node
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.3 mg/ml.
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缓冲液
- 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- CD22 (CD22 Molecule (CD22))
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别名
- CD22
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基因ID
- 933
抗原
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