RNA Polymerase II 抗体 (pSer5) (CF®594)
This anti-RNA Polymerase II antibody is a 小鼠 单克隆 antibody detecting RNA Polymerase II in WB, IHC (fp) 和 IHC. Suitable for 人, 小鼠 和 大鼠.
Quick Overview for RNA Polymerase II 抗体 (pSer5) (CF®594) (ABIN7659657)
抗原
RNA Polymerase II
适用
人, 小鼠, 大鼠
宿主
小鼠
克隆类型
单克隆
标记
This RNA Polymerase II antibody is conjugated to CF®594
应用范围
Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunohistochemistry (IHC)
克隆位点
CTD4H8
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抗原表位
- pSer5
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原理
- RNA Polymerase II(CTD4H8), CF594 conjugate
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产品特性
- RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1, 840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
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亚型
- IgG1, kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Positive Control: HAP1, K562, PC3, HePG2, NIH3T3 cells. Human testis.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- RNA Polymerase II
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背景
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Synonyms: DNA-directed RNA polymerase II largest subunit RNA polymerase II 220kd subunit, DNA-directed RNA polymerase II subunit RPB1, hRPB220, hsRPB1, POLR2, Polr2a, POLRA, Polymerase (RNA) II (DNA directed) polypeptide A 220 kDa, RNA polymerase II subunit B1, RPBh1, RpIILS, RPO2, RPOL2
Gene Symbol: POLR2A
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分子量
- 192-253 kDa
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基因ID
- 5430
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UniProt
- P24928
抗原
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