MILK FAT GLOBULIN 抗体 (CF®740)
This anti- antibody is a 小鼠 单克隆 antibody detecting in IF, IHC, IHC (fp) 和 WB. Suitable for 人.
Quick Overview for MILK FAT GLOBULIN 抗体 (CF®740) (ABIN7657115)
抗原
MILK FAT GLOBULIN
适用
人
宿主
小鼠
克隆类型
单克隆
标记
CF®740
应用范围
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Western Blotting (WB)
克隆位点
MFG-06
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原理
- Milk Fat Globulin(MFG-06), CF740 conjugate
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产品特性
- Recognizes a protein of 40-45 kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Human milk fat globule membrane preparation
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亚型
- IgG1, kappa
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anti-MILK FAT GLOBULIN antibody
适用: 人 IF, FACS, IHC (f) 宿主: 小鼠 Monoclonal EDM45 unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Normal breast or breast carcinoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- MILK FAT GLOBULIN
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别名
- Milk-Fat-Globulin
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背景
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Synonyms: Breast epithelial antigen BA46, EDIL1, HMFG, Lactadherin, Lactadherin precursor, Medin, MFGE8, MFGM, Milk fat globule-EGF factor 8 (MFG-E8)
Gene Symbol: MFGE8
Tissue Expression: Epithelial cells|Mammary gland
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分子量
- 45 kDa
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基因ID
- 4240
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UniProt
- Q08431
抗原
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