Calcineurin A 抗体 (CF®405S)
Quick Overview for Calcineurin A 抗体 (CF®405S) (ABIN7650357)
抗原
See all Calcineurin A (CAN) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Calcineurin A / PPP3CA(BF1.1), CF405S conjugate
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产品特性
- This antibody recognizes the Calcineurin A alpha protein, encoded by the PPP3CA (Protein Phosphatase 3 Catalytic Subunit Alpha) gene. Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59 kDa catalytic A subunit and a 19 kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length Calcineurin A protein
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亚型
- IgG1, kappa
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anti-Calcineurin A (CAN) (AA 15-80) antibody
CAN 适用: 小鼠, 大鼠 WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Calcineurin A (CAN) (Center) antibodyCAN 适用: 人 ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Calcineurin A (CAN) antibodyKD Validated CAN 适用: 人, 小鼠, 大鼠 WB, ICC, FACS, IF 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
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应用备注
- For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry
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说明
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Positive Control: HeLa, MCF-7 or A431 cells. Prostate, Thyroid or Pancreas.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Calcineurin A (CAN)
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别名
- Calcineurin A
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背景
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Synonyms: Calcineurin A, Calcineurin A alpha, PPP3CA, Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, CALN, CALNA1, CAM-PRP catalytic subunit, CCN1, CNA alpha, CNA1, Protein phosphatase 3 catalytic subunit alpha isoform
Gene Symbol: PPP3CA
Tissue Expression: Brain|T-cells
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分子量
- 62 kDa
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基因ID
- 5530
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UniProt
- Q08209
抗原
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