BRD4 抗体 (Middle Region)
Quick Overview for BRD4 抗体 (Middle Region) (ABIN7603026)
抗原
See all BRD4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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原理
- Anti-BRD4 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-BRD4 Antibody Picoband® (ABIN7603026). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human BRD4, which shares 95% amino acid (aa) sequence identity with mouse BRD4.
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亚型
- IgG
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anti-Bromodomain Containing 4 (BRD4) antibody
BRD4 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Bromodomain Containing 4 (BRD4) (N-Term) antibodyBRD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Bromodomain Containing 4 (BRD4) antibodyBRD4 适用: 人 WB, ELISA, IHC 宿主: 兔 Monoclonal 1E5 unconjugated Recombinant Antibody
anti-Bromodomain Containing 4 (BRD4) (AA 1313-1342), (C-Term) antibodyBRD4 适用: 人 IHC (p) 宿主: 兔 Polyclonal RB3422 unconjugated
anti-Bromodomain Containing 4 (BRD4) (C-Term) antibodyBRD4 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 兔, 马 WB, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Bromodomain Containing 4 (BRD4) (AA 150-200) antibodyBRD4 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Bromodomain Containing 4 (BRD4) (AA 1-30), (N-Term) antibodyBRD4 适用: 人 IHC (p) 宿主: 兔 Polyclonal RB3419 unconjugated
anti-Bromodomain Containing 4 (BRD4) (AA 651-700) antibodyBRD4 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 兔, 马, Pig, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Bromodomain Containing 4 (BRD4) (AA 700-794) antibodyBRD4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Bromodomain Containing 4 (BRD4) antibodyBRD4 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Rat
1. Choe, J., Lin, S., Zhang, W., Liu, Q., Wang, L., Ramirez-Moya, J., Du, P., Kim, W., Tang, S., Sliz, P., Santisteban, P., George, R. E., Richards, W. G., Wong, K.-K., Locker, N., Slack, F. J., Gregory, R. I. mRNA circularization by METTL3-eIF3h enhances translation and promotes oncogenesis. Nature 561: 556-560, 2018. 2. Crawford, N. P. S., Alsarraj, J., Lukes, L., Walker, R. C., Officewala, J. S., Yang, H. H., Lee, M. P., Ozato, K., Hunter, K. W. Bromodomain 4 activation predicts breast cancer survival. Proc. Nat. Acad. Sci. 105: 6380-6385, 2008. 3. Dawson, M. A., Prinjha, R. K., Dittmann, A., Giotopoulos, G., Bantscheff, M., Chan, W.-I., Robson, S. C., Chung, C., Hopf, C., Savitski, M. M., Huthmacher, C., Gudgin, E., and 15 others. Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukaemia. Nature 478: 529-533, 2011. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- BRD4 (Bromodomain Containing 4 (BRD4))
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别名
- BRD4
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背景
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Synonyms: Fibroblast growth factor 2, FGF-2, Basic fibroblast growth factor, bFGF, Heparin-binding growth factor 2, HBGF-2, FGF2, FGFB
Tissue Specificity: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue.
Background: Bromodomain-containing protein 4 is a protein that in humans is encoded by the BRD4 gene. The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15,19)(q13,p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
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分子量
- 220 kDa
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基因ID
- 23476
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UniProt
- O60885
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途径
- Chromatin Binding, SARS-CoV-2 Protein Interactome
抗原
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