Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
原理
Anti-SLD5/GINS4 Antibody Picoband®
产品特性
Anti-SLD5/GINS4 Antibody Picoband® (ABIN7599109). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
纯化方法
Immunogen affinity purified.
免疫原
E.coli-derived human SLD5/GINS4 recombinant protein (Position: M1-I223). Human GINS4 shares 87.9% and 88.8% amino acid (aa) sequence identity with mouse and rat GINS4, respectively.
Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry, 2-5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/mL, - 1. Kong, L., Ueno, M., Itoh, M., Yoshioka, K., Takakura, N. Identification and characterization of mouse PSF1-binding protein, SLD5. Biochem. Biophys. Res. Commun. 339: 1204-1207, 2006. 2. Takayama, Y., Kamimura, Y., Okawa, M., Muramatsu, S., Sugino, A., Araki, H. GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Genes Dev. 17: 1153-1165, 2003. 3. Ueno, M., Itoh, M., Kong, L., Sugihara, K., Asano, M., Takakura, N. PSF1 is essential for early embryogenesis in mice. Molec. Cell Biol. 25: 10528-10532, 2005.
限制
仅限研究用
状态
Lyophilized
溶解方式
Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
The yeast heterotetrameric GINS complex is made up of Sld5, Psf1 (GINS1, MIM 610608), Psf2 (GINS2, MIM 610609), and Psf3 (GINS3, MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.