Recombinant SARS-CoV-2 Spike S1 抗体
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Quick Overview for Recombinant SARS-CoV-2 Spike S1 抗体 (ABIN7566482)
抗原
See all SARS-CoV-2 Spike S1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- anti-SARS-CoV-2 Spike Protein S1 (NTD), mAb (rec.) (AB72-1-H10)
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产品特性
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Recombinant Antibody. Recognizes the SARS-CoV-2 S1 (N-terminal domain). Does not cross-react with HCoV-OC43, HCoV-229E, HCoV-NL63, HCoV-HKU1, MERS-CoV or SARS-CoV. Applications: ELISA. Clone: AB72-1-H10. Isotype: Mouse IgG2a. Formulation: Liquid. In PBS. Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many other wild animals. Recently, a new severe acute respiratory syndrome beta-coronavirus called SARS-CoV-2 (or 2019-nCoV) has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19). SARS-CoV-2 shares 79.5 % sequence identity with SARS-CoV and is 96.2 % identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S protein plays a key role in viral infection and pathogenesis. The S1 subunit contains the N-terminal domain (NTD) and a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells, whereas S2 harbors heptad repeat 1 (HR1) and HR2. The RBD domain first binds its receptor to form an RBD/ACE2 complex. This triggers conformational changes in the S protein, leading to membrane fusion mediated via HR1 and HR2 and consequently in viral entry into target cells. Antibodies targeting various regions of S protein have different mechanisms in inhibiting SARS-CoV-2 infection. For example, NTD-targeting antibodies bind the NTD to form an NTD/mAb complex, thereby preventing conformational changes in the S protein and blocking membrane fusion and viral entry. RBD-targeting antibodies form RBD/mAb or RBD/Nb complexes that inhibit binding of the RBD to ACE2, thereby preventing entry of SARS-CoV-2 into target cells.
Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many other wild animals. Recently, a new severe acute respiratory syndrome beta-coronavirus called SARS-CoV-2 (or 2019-nCoV) has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19). SARS-CoV-2 shares 79.5 % sequence identity with SARS-CoV and is 96.2 % identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S protein plays a key role in viral infection and pathogenesis. The S1 subunit contains the N-terminal domain (NTD) and a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells, whereas S2 harbors heptad repeat 1 (HR1) and HR2. The RBD domain first binds its receptor to form an RBD/ACE2 complex. This triggers conformational changes in the S protein, leading to membrane fusion mediated via HR1 and HR2 and consequently in viral entry into target cells. Antibodies targeting various regions of S protein have different mechanisms in inhibiting SARS-CoV-2 infection. For example, NTD-targeting antibodies bind the NTD to form an NTD/mAb complex, thereby preventing conformational changes in the S protein and blocking membrane fusion and viral entry. RBD-targeting antibodies form RBD/mAb or RBD/Nb complexes that inhibit binding of the RBD to ACE2, thereby preventing entry of SARS-CoV-2 into target cells.
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纯化方法
- Puified
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纯度
- >95 % (SDS-PAGE)
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免疫原
- Recombinant SARS-CoV-2 S1/S2 Protein (aa 14-1208 (proline substitutions at residues 986 and 987, ""GSAS"" substitution at the furin cleavage site (residues 682-685)) containing a C-terminal His-tag.
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亚型
- IgG2a
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anti-SARS-CoV-2 Spike S1 (RBD) antibody
适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV), SARS CoV-2 Alpha, SARS CoV-2 Beta, SARS CoV-2 Epsilon, SARS CoV-2 Gamma, SARS CoV-2 Eta, SARS CoV-2 Kappa, SARS CoV-2 Delta, SARS CoV-2 Omicron ELISA, Crys, SPR, IF 宿主: Human Monoclonal CR3022 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike S1 (RBD) antibody适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV), SARS CoV-2 Alpha, SARS CoV-2 Beta, SARS CoV-2 Epsilon, SARS CoV-2 Gamma, SARS CoV-2 Eta, SARS CoV-2 Kappa ELISA, Crys, SPR, IF 宿主: 兔 Chimeric CR3022 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike S1 antibody适用: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha, SARS CoV-2 Beta, SARS CoV-2 Epsilon, SARS CoV-2 Gamma ELISA, Neut 宿主: Human Chimeric AM122 unconjugated
anti-SARS-CoV-2 Spike S1 (RBD) antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA 宿主: 兔 Polyclonal unconjugated
anti-SARS-CoV-2 Spike S1 (RBD) antibody适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV) ELISA, Crys, SPR, Neut 宿主: Human Monoclonal CR3022 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike S1 (RBD) antibody适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV) ELISA, Crys, SPR, IF 宿主: Human Monoclonal CR3022 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike S1 (RBD) antibody适用: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV) ELISA, Crys, SPR, IF 宿主: Human Monoclonal CR3022 unconjugated Recombinant Antibody
anti-SARS-CoV-2 Spike S1 antibody适用: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha ELISA, IHC 宿主: 兔 Monoclonal SP185 unconjugated
anti-SARS-CoV-2 Spike S1 (Trimer) antibody适用: SARS Coronavirus-2 (SARS-CoV-2) ELISA, WB, LF 宿主: Cell culture Monoclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- In PBS.
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注意事项
- After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.Please handle under sterile conditions to avoid contamination.
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储存条件
- 4 °C,-20 °C
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储存方法
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Stable for at least 1 year after receipt when stored at -20°C.
Stable for at least 3 months after receipt when stored at +4°C.
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- SARS-CoV-2 Spike S1
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别名
- SARS-CoV-2 Spike Protein S1
抗原
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