SARS-CoV-2 NSP8 抗体
(1 reference)
Quick Overview for SARS-CoV-2 NSP8 抗体 (ABIN7540672)
抗原
See all SARS-CoV-2 NSP8 (NSP8) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-401-a53
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Supplier
- Rockland
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原理
- Sars-Cov Nonstructural Protein 8 Antibody
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交叉反应 （详细）
- This antibody is directed against SARS-Coronavirus nsp8 protein.
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纯化方法
- The product is neat antiserum.
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过滤
- Sterile filtered
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免疫原
- This whole rabbit serum was produced by repeated immunizations with a purified His- tagged recombinant protein corresponding to full-length SARS-Coronavirus nsp8.
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亚型
- IgG
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anti-SARS-CoV-2 Non-Structural Protein 8 (NSP8) antibody
NSP8 适用: SARS Coronavirus-2 (SARS-CoV-2) WB 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Immunoprecipitation_Dilution: 1:60
IF_Microscopy_Dilution: 1:300
Western_Blot_Dilution: 1:1,000
Other: IMMUNOELECTRON MICROSCOPY 1:100
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说明
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This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kDa in size corresponding to SARS-CoV nsp8 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37°C. Infection occurred in PBS/DEAE/2% FCS followed by exchange to EMEM/25mM HEPES/2% FCS. Cells were fixed with PBS/3% PFA. After washing fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: None
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Expression Profile and Localization of SARS-CoV-2 Nonstructural Replicase Proteins in Infected Cells." in: Microbiology spectrum, Vol. 10, Issue 4, pp. e0074422, (2022) (PubMed).
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: "Expression Profile and Localization of SARS-CoV-2 Nonstructural Replicase Proteins in Infected Cells." in: Microbiology spectrum, Vol. 10, Issue 4, pp. e0074422, (2022) (PubMed).
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- SARS-CoV-2 NSP8 (NSP8) (SARS-CoV-2 Non-Structural Protein 8 (NSP8))
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别名
- SARS-CoV-2 NSP8
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背景
- Rabbit anti-Sars-Cov Nonstructural Protein 8 Antibody, Replicase polyprotein 1a, ORF1a polyprotein, nsp8,The nonstructural protein 8 (nsp8) is one of the SARS-Coronavirus replicase cleaving products, encoded by ORF1a. Nsp8 is thought to be part of the viral replication complex, which is associated with intracellular membranes. No specific information on the function of nsp8 is available. Anti-SARS-CoV Nonstructural Protein 8 (nsp8) Antibody is useful for researchers interested in viral research.
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基因ID
- 1489680, 30124074
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UniProt
- P0C6U8
抗原
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