Recombinant Prosaposin 抗体
Quick Overview for Recombinant Prosaposin 抗体 (ABIN7477571)
抗原
See all Prosaposin (PSAP) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
免疫原
- Recombinant full-length human ACPP protein
-
亚型
- IgG
-
-
-
-
应用备注
- IHC 1:100-1:200
-
说明
-
Positive Control: Prostate: A strong PSAP immunostaining should, be seen in all glandular cells.
Negative Control: Appendix: All epithelial and non-epithelial structures must not show any PSAP immunostaining.
-
实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
-
- Prosaposin (PSAP)
-
别名
- PSAP
-
背景
- 5'-nucleotidase (5'-NT), Acid phosphatase prostate, ACP3, Ecto-5'-nucleotidase, Prostatic acid phosphatase (PAP), Prostatic acid phosphatase, Thiamine monophosphatase (TMPase),PSAP antibody validated for immunohistochemistry on 76 different Normal Tissues
-
UniProt
- P15309
-
途径
- Positive Regulation of Endopeptidase Activity
抗原
-