Recombinant Chromogranin A 抗体
Quick Overview for Recombinant Chromogranin A 抗体 (ABIN7477423)
抗原
See all Chromogranin A (CHGA) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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免疫原
- Recombinant full-length human chromogranin A protein
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亚型
- IgG
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anti-Chromogranin A (CHGA) (AA 15-148) antibody
CHGA 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chromogranin A (CHGA) (AA 24-154) antibodyCHGA 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chromogranin A (CHGA) antibodyCHGA 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CGA-413 unconjugated
anti-Chromogranin A (CHGA) (AA 278-457) antibodyCHGA 适用: 人 FACS, IHC, WB, ELISA 宿主: 小鼠 Monoclonal 5H10B12 unconjugated
anti-Chromogranin A (CHGA) (AA 87-252) antibodyCHGA 适用: 人 FACS, WB, ELISA, ICC 宿主: 小鼠 Monoclonal 1A6B6 unconjugated
anti-Chromogranin A (CHGA) (AA 17-446) antibodyCHGA 适用: Pig IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chromogranin A (CHGA) (AA 278-457) antibodyCHGA 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 1B7A4 unconjugated
anti-Chromogranin A (CHGA) antibodyCHGA 适用: 人, 小鼠 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Chromogranin A (CHGA) antibodyCHGA 适用: 人, 大鼠, 小鼠, 猴, Pig IHC, StM 宿主: 小鼠 Monoclonal LK2H10 unconjugated
anti-Chromogranin A (CHGA) antibodyCHGA 适用: 人 IHC, StM 宿主: 兔 Monoclonal CHGA-1731R unconjugated Recombinant Antibody
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Appendix: An at least weak to moderate staining must be seen in the axons and ganglion cells of the peripheral nerves while mucosal neuroendocrine cells exhibit a strong staining (neuroendocrine cells are not sufficient as positive controls because they express CGA so strongly, that even inefficient staining will detect its expression)
Negative Control: Appendix: Epithelial and smooth muscle cells should be negative.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Chromogranin A (CHGA)
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别名
- Chromogranin A
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背景
- Beta-Granin, CGA, CHGA, Chromogranin A Parathyroid Secretory Protein 1, ER-37, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I, Vasostatin I or II,Chromogranin A antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P10645
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途径
- Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
抗原
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