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ECM1 抗体

This anti-ECM1 antibody is a 小鼠 单克隆 antibody detecting ECM1 in IHC 和 IF. Suitable for 人 和 大鼠.
产品编号 ABIN6252531
发货至: 中国

Quick Overview for ECM1 抗体 (ABIN6252531)

抗原

See all ECM1 抗体
ECM1 (Extracellular Matrix Protein 1 (ECM1))

适用

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人, 大鼠

宿主

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  • 1
小鼠

克隆类型

  • 35
  • 31
单克隆

标记

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This ECM1 antibody is un-conjugated

应用范围

  • 42
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  • 10
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Immunohistochemistry (IHC), Immunofluorescence (IF)

克隆位点

SC05
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    Secretory component protein isolated from human colostrum was used as the immunogen.

    亚型

    IgG1 kappa
  • 应用备注

    IF: 1-2 μg/mL

    IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    ECM1 (Extracellular Matrix Protein 1 (ECM1))

    别名

    Extracellular Matrix Protein 1

    背景

    This antibody reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory component. It does not react with the cell lines lacking this protein. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

    基因ID

    1893

    UniProt

    Q16610
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