Proximal-Nephrogenic Antigen / Renal Cell Carcinoma (RCC) 抗体 (CF®647)
This anti- antibody is a 小鼠 单克隆 antibody detecting in FACS, IF, IHC (f) 和 WB. Suitable for 马 和 人.
Quick Overview for Proximal-Nephrogenic Antigen / Renal Cell Carcinoma (RCC) 抗体 (CF®647) (ABIN6153727)
抗原
Proximal-Nephrogenic Antigen / Renal Cell Carcinoma (RCC)
适用
马, 人
宿主
小鼠
克隆类型
单克隆
标记
CF®647
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
克隆位点
66-4-C2
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原理
- Mouse Monoclonal anti-Proximal Nephrogenic Antigen / Renal Cell Carcinoma (RCC) (66.4.C2), CF647 Conjugate
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产品特性
- This antibody recognizes a glycoprotein of ~200 kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Microsomal fraction of human renal cortical tissue homogenate
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亚型
- IgG2b kappa
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anti-Proximal-Nephrogenic Antigen / Renal Cell Carcinoma (RCC) antibody
适用: 马, 人 FACS, IF, IHC (f), WB 宿主: 小鼠 Monoclonal 66-4-C2 unconjugated
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应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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Normal kidney or renal cell carcinoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- Proximal-Nephrogenic Antigen / Renal Cell Carcinoma (RCC)
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分子量
- 55 kDa
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基因ID
- 768, 63287
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UniProt
- Q16790
抗原
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