Thyroglobulin 抗体
Quick Overview for Thyroglobulin 抗体 (ABIN5707703)
抗原
See all Thyroglobulin (TG) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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交叉反应 (详细)
- Not suitable for mouse
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纯化方法
- Purified
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纯度
- Protein A affinity chromatography
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免疫原
- Human thyroid follicular cells were used as the immunogen for the recombinant TG antibody.
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亚型
- IgG kappa
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应用备注
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The stated application concentrations are suggested starting points. Titration of the recombinant TG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.1-0.2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1) -
限制
- 仅限研究用
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant TG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Thyroglobulin (TG)
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别名
- TG / Thyroglobulin
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背景
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Thyroglobulin (Tg) is a 660 kDa, dimeric protein produced by the follicular cells of the thyroid and used entirely within the thyroid gland. Thyroglobulin protein accounts for approximately half of the protein content of the thyroid gland. Human TG (HTG) is a homodimer of subunits each containing 2768 amino acids as synthesized (a short signal peptide may be removed from the N-terminus in the mature protein).
The protein is a precursor of the thyroid hormones, these are produced when thyroglobulin's tyrosine residues are combined with iodine and the protein is subsequently cleaved. Each thyroglobulin molecule contains approximately 100-120 tyrosine residues, but only a small number (20) of these are subject to iodination by thyroperoxidase in the follicular colloid. Therefore, each Tg molecule forms only approximately 10 thyroid hormone molecules. [Wiki]
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途径
- Thyroid Hormone Synthesis
抗原
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