Protein A 抗体 (Texas Red (TR))
Quick Overview for Protein A 抗体 (Texas Red (TR)) (ABIN401017)
抗原
See all Protein A 抗体适用
宿主
克隆类型
标记
应用范围
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产品特性
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Absorption / Emission: 596 nm / 620 nm
Molar Ratio: 1.5 moles Texas Red(TM) per mole of Goat IgG -
纯化方法
- Multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
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免疫原
- Protein A [Staphylococcus aureus]
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亚型
- IgG
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应用备注
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Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring lot-to-lot consistency. Flow cytometry: 1: 2,000 - 1: 10,000. Immunoflourescence: 1: 500 - 1: 2,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
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浓度
- 10.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 as buffer, 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer, 0.01 % (w/v) Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for two weeks at 2-8° C as an undiluted liquid. Dilute only prior to immediate use.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store vial at 2-8° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below.
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- Protein A
抗原
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