Fc epsilon Receptor 1 抗体
适用: 人
WB, ELISA, FACS, IP, IF, IHC, IHC (p)
宿主: 小鼠
Monoclonal
9E1
unconjugated
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- 抗原
- Fc epsilon Receptor 1
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Recognizes the human high affinity Fc receptor for IgE (Fc epsilon R1), which plays a central role in the IgE-mediated allergic response. Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two disulfide linked gamma subunits. Clone Recognizes the extracellular D1 domain of the Fc epsilon R1 alpha chain. Fc epsilon R1 is primarily expressed on mast cells and basophils but expression of Fc epsilon R1 has also been reported on monocytes, Langerhans cells and dendritic cells from patients with atopic diseases.
- 纯化方法
- Affinity purified
- 免疫原
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Type of Immunogen: Native protein - 克隆位点
- 9E1
- 亚型
- IgG2b
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anti-Fc epsilon Receptor 1 antibody (PE)
适用: 人 WB, ELISA, FACS, IP, IF, IHC, IHC (p) 宿主: 小鼠 Monoclonal 9E1 PE
anti-Fc epsilon Receptor 1 antibody适用: 人 WB, ELISA, FACS, IP, IF, IHC 宿主: 小鼠 Monoclonal 9-00E-001 unconjugated
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- 应用备注
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Approved: ELISA, Flo (1:25 - 1:50), IF, IHC, IHC-P (10 μg/mL), IP, WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.4, 0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- 抗原
- Fc epsilon Receptor 1
- 背景
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Name/Gene ID: FceR1
Synonyms: FceR1
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