FBXO43 抗体 (C-Term)
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Quick Overview for FBXO43 抗体 (C-Term) (ABIN374351)
抗原
See all FBXO43 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-PGSAQSKRNL KRL
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特异性
- Recognizes EMI2.
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交叉反应 (详细)
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Species reactivity (expected):Human, Mouse, Canine, Pig, Cow.
Species reactivity (tested):Rat. -
纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide C Terminus of the protein sequence according to NP_001025031.2. Genename: FBXO43
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应用备注
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Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-3 μg/mL. Has been successfully used on Rat Pachytene Spermatocytesshowing a 71 kDa band (Perrard et al, PLoS One.2009 Oct 5,4(10): e7237. PMID: 19802389). This product has been successfully used in Immunofluorescence and Flow Cytometry onRat meiotic Testis cells (5-10 μg/mL): (Perrard et al, PLoS One.2009 Oct 5,4(10): e7237. PMID: 19802389).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- FBXO43 (F-Box Protein 43 (FBXO43))
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别名
- FBXO43
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背景
- Members of the F-box protein family, such as Fbp5B, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1, MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains.Synonyms: EMI2, Endogenous meiotic inhibitor 2, F-box only protein 43, FBP5B
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基因ID
- 286151
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NCBI登录号
- NP_001025031
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UniProt
- Q4G163
抗原
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