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EpCAM 抗体 (FITC)

This anti-EpCAM antibody is a 小鼠 单克隆 antibody detecting EpCAM in FACS, IF 和 IHC (fro). Suitable for 人.
产品编号 ABIN356311
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for EpCAM 抗体 (FITC) (ABIN356311)

抗原

See all EpCAM (EPCAM) 抗体
EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

适用

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宿主

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小鼠

克隆类型

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单克隆

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This EpCAM antibody is conjugated to FITC

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

ESA214
  • 特异性

    This antibody has been used recently to characterize the EpCAM high CD44+ versus EpCAM low/Cd44- indifferent cancer cells.
    Cellular Localization: Cell membrane.

    产品特性

    Synonyms: Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2,M4S1, MIC18, Tumor-associated calcium signal transducer 1, Major gastrointestinaltumor-associated protein GA733-2, Epithelial glycoprotein 314,Adenocarcinoma-associated antigen, KS 1/4 antigen

    纯化方法

    Protein A Chromatography.

    免疫原

    Small cell lung carcinoma. Remarks: Molecular weight of antigen: 40 kDa.

    亚型

    IgG1
  • 应用备注

    Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. Antigen retriever like pepsin enhances the staining of FFPE tissue. Immunofluorescence: 10-20 μg/mL (1/10-1/20), incubate for 2 hours in the dark at RT or itcan also be incubated overnight at 4 °C. Flow Cytometry: 0.2-1.0 μg/0.1 mL(1/200-1/1,000). Recommended Positive Control: Breast carcinoma.

    限制

    仅限研究用
  • 浓度

    0.2 mg/mL

    缓冲液

    PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
    Shelf life: One year from despatch.

    有效期

    12 months
  • 抗原

    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

    别名

    CD326 / EPCAM / TACSTD1

    背景

    Epithelial is a transmembrane glycoprotein (40 kDa) also known as ESA or epithelial cellular adhesion molecule (Ep-CAM). This protein is expressed on basolateral cell surface in most simple epithelia and vast majority of cancers. According to the Synonyms: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1

    基因ID

    4072

    UniProt

    P16422
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