Coat Protein g8p 抗体
Cited in 1 publication. This 小鼠 单克隆 anti-not_set antibody (Clone RL-ph1) specifically detects not_set in AC, ICC, FACS, IHC, ELISA 和 WB. The antibody is reactive with Bacteriophage M13 samples.
1 image
(1 reference)
产品编号 ABIN335342
发货至:
中国
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Quick Overview for Coat Protein g8p 抗体 (ABIN335342)
抗原
Coat Protein g8p
适用
Bacteriophage M13
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Affinity Chromatography (AC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
克隆位点
RL-ph1
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纯化方法
- Purified
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免疫原
- RL-ph1 is a mouse monoclonal IgG2b, kappa antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with isolated M13 phage coat proteins.
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亚型
- IgG2b
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anti-Coat Protein g8p antibody
适用: Bacteriophage M13 AC, ICC, FACS, IHC, ELISA, WB 宿主: 小鼠 Monoclonal RL-ph2 unconjugated
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应用备注
- RL-ph1 reacts with the major M13 filamentous phage coat protein g8p with a molecular weight of 5 kDa. RL-ph1 is particularly useful for immunoblotting of separated phage proteins, and is also suitable for immunocytochemistry, flow cytometry, affinity chromatography and ELISA. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
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限制
- 仅限研究用
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储存条件
- 4 °C
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: "Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody." in: Human antibodies and hybridomas, Vol. 6, Issue 3, pp. 113-8, (1996) (PubMed).
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: "Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody." in: Human antibodies and hybridomas, Vol. 6, Issue 3, pp. 113-8, (1996) (PubMed).
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- Coat Protein g8p
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物质类
- Phage Protein
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背景
- The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
抗原
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