CD3 抗体 (Cytoplasmic Domain) (FITC)
(1 reference)-
- 抗原 See all CD3 抗体
- CD3
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抗原表位
- Cytoplasmic Domain
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适用
- 人, 小鸡, 猴, Pig, 犬, 马, Cow
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CD3 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS)
- 序列
- Glu-Arg-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys
- 特异性
- This antibody recognises a highly conserved epitope of the CD3 Molecule expressed by T lymphocytes. Cross reacts with Bovine, Dog, Horse, Rhesus Monkey, Pig, Mouse and Chicken.
- 纯化方法
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- 免疫原
- Synthetic peptide sequence derived from cytoplasmic epitope of CD3.
- 克隆位点
- CD3-12
- 亚型
- IgG1
- Top Product
- Discover our top product CD3 Primary Antibody
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anti-CD3 (CD3) antibody
CD3 适用: 人, 小鼠, 大鼠 IHC, IF, WB 宿主: 兔 Polyclonal unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), ICC, CyTOF 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, CyTOF 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, Func, CyTOF 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), Func, CyTOF 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-57 Biotin
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal UCHT1 Biotin
anti-CD3 (CD3) antibody (PerCP)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PerCP
anti-CD3 (CD3) antibody (PerCP)CD3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-57 PerCP
anti-CD3 (CD3) antibody (APC)CD3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-57 APC
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- 应用备注
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Flow Cytometry: Use 10 μL of 1/5-1/10 diluted antibody to label 10^6 cells in 100 μL. Membrane permeabilisation is required. We recommend use of Leucoperm for this purpose.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.1 mg/mL
- 缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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: "B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 4819-26, (2010) (PubMed).
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: "B cell-derived vascular endothelial growth factor A promotes lymphangiogenesis and high endothelial venule expansion in lymph nodes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 9, pp. 4819-26, (2010) (PubMed).
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- 抗原
- CD3
- 别名
- CD3 (CD3 产品)
- 背景
- T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
- 基因ID
- 916
- NCBI登录号
- NP_000724
- UniProt
- P07766
- 途径
- TCR Signaling, Ubiquitin Proteasome Pathway
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