Netrin 1 抗体 (N-Term)
(2 references)Quick Overview for Netrin 1 抗体 (N-Term) (ABIN3044248)
抗原
See all Netrin 1 (NTN1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 32-51, N-Term
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原理
- Anti-Netrin 1/NTN1 Antibody Picoband®
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序列
- AGQAAQPDPC SDENGHPRRC
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Netrin 1/NTN1 Antibody (ABIN3044248). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human Netrin 1, identical to the related rat and mouse sequences.
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亚型
- IgG
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anti-Netrin 1 (NTN1) (C-Term) antibody
Verified NTN1 适用: 人 ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Netrin 1 (NTN1) (AA 501-604) antibodyNTN1 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Netrin 1 (NTN1) (C-Term) antibodyNTN1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Netrin 1 (NTN1) (AA 181-195), (Secreted) antibodyNTN1 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Netrin 1 (NTN1) (C-Term) antibodyNTN1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Netrin 1 (NTN1) (AA 186-478) antibodyNTN1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Netrin 1 (NTN1) (C-Term) antibodyNTN1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Netrin 1 (NTN1) antibodyMicroarray verified NTN1 适用: 人 IHC 宿主: 小鼠 Monoclonal MAB935839 unconjugated
anti-Netrin 1 (NTN1) antibodyMicroarray verified NTN1 适用: 人 IHC 宿主: 小鼠 Monoclonal MAB577874 unconjugated
anti-Netrin 1 (NTN1) (C-Term) antibodyNTN1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Colon-Ramos, D. A., Margeta, M. A., Shen, K. Glia promote local synaptogenesis through UNC-6 (netrin) signaling in C. elegans. Science 318: 103-106, 2007. 2. Corset, V., Nguyen-Ba-Charvet, K. T., Forcet, C., Moyse, E., Chedotal, A., Mehlen, P. Netrin-1-mediated axon outgrowth and cAMP production requires interaction with adenosine A2b receptor. Nature 407: 747-750, 2000. 3. Powell AW, Sassa T, Wu Y, Tessier-Lavigne M, Polleux F (May 2008). Ghosh, Anirvan. ed. "Topography of thalamic projections requires attractive and repulsive functions of Netrin-1 in the ventral telencephalon." -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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: "Reduced expression of netrin-1 is associated with fetal growth restriction." in: Molecular and cellular biochemistry, Vol. 350, Issue 1-2, pp. 81-7, (2011) (PubMed).
: "Effect of different concentrations of neogenin on proliferation, apoptosis and related proliferative factors in human trophoblasts." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 30, Issue 4, pp. 500-4, (2010) (PubMed).
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: "Reduced expression of netrin-1 is associated with fetal growth restriction." in: Molecular and cellular biochemistry, Vol. 350, Issue 1-2, pp. 81-7, (2011) (PubMed).
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- Netrin 1 (NTN1)
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别名
- NTN1
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背景
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Synonyms: Netrin-1,Epididymis tissue protein Li 131P,NTN1,NTN1L,
Tissue Specificity: Widely expressed in normal adult tissues with highest levels in heart, small intestine, colon, liver and prostate. Reduced expression in brain tumors and neuroblastomas. Expressed in epididymis (at protein level). .
Background: Netrin-1 (NTN1), also known as NTN1L, is a diffusible protein made by floor plate cells. Netrin-1 is found in the floor plate and neuroepithelial cells of the ventral region of the spinal cord, as well as other locations in the nervous system including the somatic mesoderm, pancreas and cardiac muscle. The human NTN1 gene is mapped to chromosome 17p13-p12 by FISH. Netrin-1 can attract spinal commissural axons and repel trochlear axons in vitro. And netrin-1 expression confers a selective advantage for tumor cell survival. The binding of netrin-1 to DCC appears to depend on the presence of a coreceptor or accessory proteins. Adenosine A2b receptor is actually a netrin-1 receptor and induces cAMP accumulation on binding netrin-1, and that netrin-1-dependent outgrowth of dorsal spinal cord axons ly involves A2b. Netrin-1 can promote intestinal tumor development, probably by regulating cell survival. Thus, a netrin-1 receptor or receptors function as conditional tumor suppressors. Expression of Unc5b triggered endothelial cell repulsion in response to netrin-1 in vitro, whereas a truncated Unc5b lacking the intracellular signaling domain failed to induce repulsion.
Sequence Similarities: Contains 3 laminin EGF-like domains.
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分子量
- 8 kDa
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UniProt
- O95631
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途径
- Regulation of Cell Size
抗原
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