The optimal dilution of the c-Myb antibody for each application should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence (1-2 μg/mL)
限制
仅限研究用
浓度
1 mg/mL
缓冲液
1 mg/mL in 1X PBS, BSA free, sodium azide free
储存液
Azide free
储存条件
4 °C,-20 °C
储存方法
Store the c-Myb antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
The highly leukemogenic avian retrovirus E26 contains two oncogenes, v-Myb and v-Ets, which are expressed together as a fusion protein. The cellular homolog of v-Myb, designated c-Myb, encodes a transcription factor. Deletion or disruption of a negative regulatory domain mapping within the carboxy-terminal domain of c-Myb results in enhanced transactivating capacity and in parallel, leads to activation of its ability to transform hemopoietic cells. c-Myb is expressed preferentially, but not exclusively, in immature hemopoietic cells and its expression decreases as cells differentiate.