Recombinant ART2.2/Art2b 抗体
This anti-ART2.2/Art2b antibody is a 小鼠 单克隆 antibody detecting ART2.2/Art2b in ICFC. Suitable for 小鼠.
产品编号 ABIN2656685
发货至:
中国
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Contact Info
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中国
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北京 101111
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Quick Overview for Recombinant ART2.2/Art2b 抗体 (ABIN2656685)
抗原
ART2.2/Art2b (Art2b)
(ADP-Ribosyltransferase 2b (Art2b))
抗体类型
Recombinant Antibody
片段
single-domain Antibody (sdAb)
适用
小鼠
宿主
小鼠
克隆类型
单克隆
标记
This ART2.2/Art2b antibody is un-conjugated
应用范围
Intracellular Flow Cytometry (ICFC)
克隆位点
S-16a
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产品特性
- ARTC2, also known as ART2.2, is a 35 kD, GPI-anchored, and cell surface ADP-ribosyltransferase that is highly expressed by regulatory T cells (Tregs) and natural killer T (NKT) cells. ARTC2 is enzymatically active at 4°C. Through the ribosylation of the ion channel P2X7, ARTC2 mediates NAD+-induced cell death (NICD) and the proteolytic shedding of CD27 and CD62L. Blocking the activity ARTC2 with Treg-Protector™ improves the recovery of Tregs and NKT cells during their isolation from lymphatic and peripheral tissues.
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纯度
- ≥ 95%, as determined by Coomassie stained SDS-PAGE.
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内毒素水平
- Less than 0.01 ng of endotoxin/µg of protein, as determined by the LAL method.
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免疫原
- Expression vector encoding full length mouse ARTC2.
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亚型
- Ig Fraction
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anti-ADP-Ribosyltransferase 2b (Art2b) antibody
Art2b 适用: 小鼠 FACS, IP 宿主: 大鼠 Monoclonal Nika102 unconjugated
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应用备注
- Treg-Protector™ (S+16a) is a recombinant llama VH Ig domain that binds to the ADP-ribosyltransferase ARTC2, which reversibly blocks its enzymatic activity and protects ARTC2+ cells from NAD+-induced cell death (NICD), which is caused by the release of NAD+ by cells that die during the preparation of primary lymphocytes. The S+16a has been demonstrated to protect a variety of cell types including Tregs, NKT cells, and tissue-resident memory T cells.
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说明
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We recommend diluting Treg-Protector™ 1:4 with sterile PBS and administer 200 µL (50 µg) intravenously (i.v.) of diluted Treg-Protector™ per mouse. Euthanize the mouse and begin the cell preparation 15 - 60 minutes after the injection. Intraperitoneal (IP) injection is also effective at similar doses (validated by developer).
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- 1 mg/mL S-16a in PBS Treg-Protector ™ is sterilized by filtration through a 0.22 μm membrane and contains no preservatives.
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储存液
- Without preservative
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注意事项
- Do not freeze. Keep sterile.
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储存条件
- 4 °C
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储存方法
- Store between 2°C and 8°C. Do not freeze. Keep sterile.
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- ART2.2/Art2b (Art2b) (ADP-Ribosyltransferase 2b (Art2b))
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别名
- ARTC2 Nanobody
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基因ID
- 11872
抗原
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