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DYKDDDDK Tag 抗体 (iFluor 488)

IF, FACS 宿主: 小鼠 Monoclonal 5A8E5 iFluor 488
产品编号 ABIN2017574
发货至: 中国
  • 抗原 See all DYKDDDDK Tag products
    DYKDDDDK Tag
    适用
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    小鼠
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    This DYKDDDDK Tag antibody is conjugated to iFluor 488
    应用范围
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    Immunofluorescence (IF), Flow Cytometry (FACS)
    品牌
    THE™
    序列
    DYKDDDDK
    产品特性
    THETM DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse is THETM DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 488 under optimal conditions with an F/P ratio of 4-8. It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 488 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 488.
    纯化方法
    Protein A affinity column
    免疫原
    A synthetic peptide (DYKDDDDK) coupled to KLH
    克隆位点
    5A8E5
    亚型
    IgG2b kappa
  • 应用备注
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed has not been determined. The following concentration ranges are recommended starting points for this product.
    Immunofluorescent staining: 1-4 μg/mL
    Flow cytometry: 1-4 μg/mL
    Other applications: user-optimized
    限制
    仅限研究用
  • 注意事项
    Avoid repeated freezing and thawing cycles.
    储存条件
    4 °C/-20 °C
    储存方法
    The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
    有效期
    12 months
  • 抗原
    DYKDDDDK Tag
    Abstract
    DYKDDDDK Tag 产品
    物质类
    Tag
    背景
    The Flag tag is a polypeptide of with the amino acid sequence DYKDDDDK amino acid sequence that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-Flag tag antibody is a useful tool for the analysis of Flag-tagged proteins.
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