UNC13A/Munc13-1 抗体 (AA 3-317)
(6 references)Quick Overview for UNC13A/Munc13-1 抗体 (AA 3-317) (ABIN1742276)
抗原
See all UNC13A/Munc13-1 (UNC13A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 3-317
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特异性
- Specific for munc 13-1.
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纯化方法
- purified IgG
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免疫原
- Recombinant rat munc 13-1 (aa 3-317).
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亚型
- IgG2b
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anti-Unc-13 Homolog A (UNC13A) (AA 3-317) antibody
UNC13A 适用: 人, 小鼠, 大鼠, 斑马鱼 WB, IP, ICC, IHC 宿主: 兔 Polyclonal unconjugated
anti-Unc-13 Homolog A (UNC13A) (AA 3-317) antibodyUNC13A 适用: 小鼠, 大鼠, 斑马鱼 WB, ICC, IHC 宿主: 豚鼠 Polyclonal unconjugated
anti-Unc-13 Homolog A (UNC13A) antibodyUNC13A 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1 : 500 up to 1 : 5000 (AP staining)
ICC: limited
IHC: not tested yet -
说明
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IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. ICC: limited to methanol fixation. ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
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缓冲液
- PBS
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注意事项
- Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
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储存条件
- -20 °C
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储存方法
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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: "Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 23, pp. 9769-80, (2013) (PubMed).
: "Different Munc13 isoforms function as priming factors in lytic granule release from murine cytotoxic T lymphocytes." in: Traffic (Copenhagen, Denmark), Vol. 14, Issue 7, pp. 798-809, (2013) (PubMed).
: "Formation of Golgi-derived active zone precursor vesicles." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 32, pp. 11095-108, (2012) (PubMed).
: "Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals." in: Journal of neurochemistry, Vol. 116, Issue 4, pp. 476-85, (2011) (PubMed).
: "The metabotropic glutamate receptor mGlu7 activates phospholipase C, translocates munc-13-1 protein, and potentiates glutamate release at cerebrocortical nerve terminals." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17907-17, (2010) (PubMed).
: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).
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: "Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 23, pp. 9769-80, (2013) (PubMed).
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- UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))
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别名
- Munc 13-1
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途径
- Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis
抗原
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