UNC13A/Munc13-1 抗体 (AA 3-317)
(6 references)Quick Overview for UNC13A/Munc13-1 抗体 (AA 3-317) (ABIN1742276)
抗原
See all UNC13A/Munc13-1 (UNC13A) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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抗原表位
- AA 3-317
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原理
- Mouse anti-Munc13-1 IgG
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特异性
- Specific for munc 13-1.
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交叉反应 （详细）
- Reacts with: rat (Q62768), mouse (Q4KUS2), zebrafish. Other species not tested yet.
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纯化方法
- purified IgG
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免疫原
- Recombinant protein corresponding to AA 3 to 317 from rat Munc13-1 (UniProt Id: Q62768)
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亚型
- IgG2b
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anti-Unc-13 Homolog A (UNC13A) (AA 3-317) antibody
KO Validated UNC13A 适用: 人, 小鼠, 大鼠, 斑马鱼 WB, ICC, IP, IHC 宿主: 兔 Polyclonal unconjugated
anti-Unc-13 Homolog A (UNC13A) (AA 3-317) antibodyKO Validated UNC13A 适用: 小鼠, 大鼠, 斑马鱼 WB, ICC 宿主: 豚鼠 Polyclonal unconjugated
anti-Unc-13 Homolog A (UNC13A) antibodyUNC13A 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB:1 : 500 up to 1 : 5000 , IP:yes ICC:not tested yet IHC:not tested yet IHC-P:not tested yet ELISA:
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说明
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IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. ICC: limited to methanol fixation. ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.
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缓冲液
- PBS. Albumin and azide were added for stabilization.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze! Antibodies should be stored at +4°C when still lyophilized.
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储存条件
- -20 °C,-80 °C,4 °C
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储存方法
- Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!
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: "Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 23, pp. 9769-80, (2013) (PubMed).
: "Different Munc13 isoforms function as priming factors in lytic granule release from murine cytotoxic T lymphocytes." in: Traffic (Copenhagen, Denmark), Vol. 14, Issue 7, pp. 798-809, (2013) (PubMed).
: "Formation of Golgi-derived active zone precursor vesicles." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 32, pp. 11095-108, (2012) (PubMed).
: "Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals." in: Journal of neurochemistry, Vol. 116, Issue 4, pp. 476-85, (2011) (PubMed).
: "The metabotropic glutamate receptor mGlu7 activates phospholipase C, translocates munc-13-1 protein, and potentiates glutamate release at cerebrocortical nerve terminals." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17907-17, (2010) (PubMed).
: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).
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: "Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 23, pp. 9769-80, (2013) (PubMed).
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- UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))
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别名
- Munc13-1
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背景
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Synonyms: Munc13-1, Munc13, Protein unc-13 homolog A, Unc13a, Unc13h1, Brain, Hippocampus, Cerebellum, Olfactory bulb, Cortex, Striatum
Antigen Information: K.O. Munc13 proteins are the mammalian homologues of the C. elegans unc-13 gene essential for exocytosis
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UniProt
- Q62768
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途径
- Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis
抗原
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