ITGAL 抗体
(3 references)Quick Overview for ITGAL 抗体 (ABIN120693)
抗原
See all ITGAL 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
特异性
- The antibody recognizes an unique epitope on CD11a antigen (LFA-1 alpha chain) implicated in activation of LFA-1 complex.
-
交叉反应 （详细）
- Species reactivity (tested):Human.
-
纯化方法
- Affinity Chromatography on Protein A
-
免疫原
- Human peripheral blood lymphocytes
-
亚型
- IgG1
-
-
anti-Integrin alpha-L (ITGAL) (AA 26-306) antibody
ITGAL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha-L (ITGAL) (AA 26-194) antibodyITGAL 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 5G7C5 unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人 FACS, IHC, IP 宿主: 小鼠 Monoclonal 38 unconjugated
anti-Integrin alpha-L (ITGAL) (AA 201-500) antibodyITGAL 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 小鼠 FACS, IP, IHC (fro) 宿主: 大鼠 Monoclonal M17-4 unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-25 unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人 FACS, IP, Func 宿主: 小鼠 Monoclonal MEM-25 unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人 FACS, IP, Func 宿主: 小鼠 Monoclonal MEM-25 unconjugated
anti-Integrin alpha-L (ITGAL) antibody (Biotin)ITGAL 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-25 Biotin
-
-
应用备注
-
Functional Application. The antibody MEM-83 directly induces the binding of T cells to purified ICAM-1. Using an in vitro-translated CDlla cDNA deletion series, the MEM-83 activation epitope was mapped to the I domain of the LFA-1 a subunit. The studies have therefore identified a novel LFA-1 activation epitope mapping to the I domain of LFA-1, which could play a role in the regulation of LFA-1 binding to ICAM-1. Flow Cytometry. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
浓度
- 1.0 mg/mL
-
缓冲液
- Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C/-20 °C
-
储存方法
-
Store the antibody at 4-8 °C for one month or at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. -
有效期
- 12 months
-
-
-
: "Mapping the binding site on CD8 beta for MHC class I reveals mutants with enhanced binding." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 6, pp. 3930-8, (2006) (PubMed).
: "Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3." in: The Journal of cell biology, Vol. 126, Issue 2, pp. 529-37, (1994) (PubMed).
: "A novel LFA-1 activation epitope maps to the I domain." in: The Journal of cell biology, Vol. 120, Issue 6, pp. 1519-27, (1993) (PubMed).
-
: "Mapping the binding site on CD8 beta for MHC class I reveals mutants with enhanced binding." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 6, pp. 3930-8, (2006) (PubMed).
-
- ITGAL (Integrin alpha-L (ITGAL))
-
别名
- CD11a / ITGAL
-
背景
- CD11a (LFA-1a) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the aLb2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.Synonyms: CD11 antigen-like family member A, Integrin alpha-L, LFA-1, LFA1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain
-
基因ID
- 3683
-
UniProt
- P20701
-
途径
- Activated T Cell Proliferation, Integrin Complex
抗原
-
